We narrowed to 8,565 results for: Dos
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Plasmid#183538PurposeDeliver a split compact Prime Editor in vivoDepositorInsertN-terminal fragment of SpCas9 nickase (H840A)
UseAAVExpressionMammalianAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Archon-EGFP
Plasmid#187979PurposeExpresses the fluorescent voltage indicator Archon under the GFAP promoterDepositorInsertArchon1-EGFP
UseAAVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmNG2(11)
bsd
UseCRISPRAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F0
Plasmid#184162PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmNG2(11)
bsd
UseCRISPRAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CC-FLAG
Plasmid#179162PurposeExpression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertprotein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
TagsFLAGExpressionMammalianAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-1
Plasmid#163137PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT2R1AtR2NtB2
Plasmid#160886PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T2
ExpressionBacterialPromoterrbcS-T2: T7, chaperones: T9Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: pBAD, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0.017
Plasmid#119621PurposeLevel 0 Part. CDSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9
UseSynthetic BiologyMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJR2953
Plasmid#122549PurposeExpression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemCardinal2
ExpressionBacterialPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC94
Plasmid#118912Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and eGFP-HIS6ExpressionInsectPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman LRRC33 and GARP chimeria (NRROS Human)
TagsFlag tagExpressionMammalianPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15α1
Plasmid#101712PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
33-G-X5-pEF1/V5-His
Plasmid#111605PurposeExpress human 33-G-X5 in 293T cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman LRRC33 and GARP chimeria (NRROS Human)
TagsFlag tagExpressionMammalianPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-5xA
Plasmid#92010PurposeExpresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
TagsLambda-N-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)Available sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 T230A 2014 EBOV + Mucin-Like Domain
Plasmid#86017PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationChanged threonine 230 to alaninePromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 D637G 2014 EBOV + Mucin-Like Domain
Plasmid#86018PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationChanged aspartic acid 637 to glycinePromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain
Plasmid#86020PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMakona Ebola Virus Glycoprotein
ExpressionMammalianMutationChanged alanine 82 to valine, threonine 230 to al…PromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSc-His6-Sem1Sc
Plasmid#83235PurposepGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeastDepositorAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only