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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 4321 - 4340 of 12282 results

  5. cycLuc_PPVs

    Plasmid
    #119208
    Purpose
    Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyclic luciferase with PPVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Promoter
    CMV
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    119208-plasmid-map-sequence-id-244622

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  6. pOCC7

    Plasmid
    #118876
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118876-plasmid-map-sequence-id-232237

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  10. TFORF1244

    Plasmid
    #143777
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PHF20 (PHF20 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143777-plasmid-map-sequence-id-396233

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  14. pJL1-MBP-4xDQNAT

    Plasmid
    #128390
    Purpose
    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Publication
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Maltose binding protein
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    A302T- please see depositor comment
    Promoter
    T7
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128390-plasmid-map-sequence-id-260183

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  15. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  19. NUDT7 (NUDT7A-c002)

    Plasmid
    #98235
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT7A (NUDT7 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98235-plasmid-map-sequence-id-193415

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  20. 13SA-csy4

    Plasmid
    #97037
    Purpose
    Spectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectors
    Depositor
    Chris Jeans
    Insert
    Cys4
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97037-plasmid-map-sequence-id-182491

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Showing: 4321 - 4340 of 12282 results