We narrowed to 9,280 results for: Pol;

1. pCsC_spacer

   Plasmid

   #136077

   Purpose

   Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCsE_spacer

   Plasmid

   #136078

   Purpose

   Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCsB

   Plasmid

   #136068

   Purpose

   Acceptor plasmid for even level position 2, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCsC

   Plasmid

   #136069

   Purpose

   Acceptor plasmid for even level position 3, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCsE

   Plasmid

   #136070

   Purpose

   Acceptor plasmid for even level position 4, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. ptrc99a-pVhb-tdTER-fdh

   Plasmid

   #215649

   Purpose

   pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   trans-enoyl-CoA reductase
   formate dehydrogenase
   

   Expression

   Bacterial

   Promoter

   Vhb

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. psicheck-2-Acsm2

   Plasmid

   #48158

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of Acsm2 (Acsm2 Mouse)
   

   Use

   Luciferase

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ABKAR(T/A)

   Plasmid

   #140052

   Purpose

   Negative control mutant for ABKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR(T/A)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC-4CL1

   Plasmid

   #35947

   Depositor

   Claudia Schmidt-dannert

   Article

   Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

   Insert

   4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLac-mod

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. p426 103Q GPD

    Plasmid

    #1184

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pPM1001

    Plasmid

    #167548

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Inaba serotype
    

    Use

    Cosmid

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMT-PT2A-iRFP670-Tdtomato-GFP

    Plasmid

    #111817

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    Tdtomato
    GFP
    

    Use

    Cloning intermediate

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRB-ECFP(W66A)-Giantin

    Plasmid

    #67903

    Purpose

    Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    FRB-ECFP(W66A)-Giantin
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFAP-Lyn-mCherry-pHluorin

    Plasmid

    #187980

    Purpose

    Membrane targeted cytoplasmic fluorescent pH sensor

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Lyn-mcherry-pHluorin
    

    Use

    AAV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046955

    Plasmid

    #145616

    Purpose

    Bacterial Expression plasmid for RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Lck-FRB-ECFP(W66A)

    Plasmid

    #67902

    Purpose

    Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    Lck-FRB-ECFP(W66A)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UbC-mCherry Rab5

    Plasmid

    #115239

    Purpose

    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab5a (RAB5A Human, Homo sapiens)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human UbC

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-NEMO

    Plasmid

    #11966

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMO (IKBKG Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pNK869

    Plasmid

    #219737

    Purpose

    MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

    Insert

    promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
    

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    AtPD7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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