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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGFP-ATIC

Plasmid

#99110

Purpose

Expresses human GFP-ATIC fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

ATIC (ATIC Human)

Tags

GFP

Expression

Mammalian

Mutation

S65T mutation in GFP gene

Promoter

CMV

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-SpvB

Plasmid

#89455

Purpose

Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.GFP

Plasmid

#59404

Purpose

expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

400 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pFOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 5

Plasmid

#51764

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 5

Use

CRISPR and Lentiviral

Promoter

EFS and U6

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

Plasmid

#40343

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

RSV promoter

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

RSV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

Plasmid

#99277

Purpose

Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mOrange2-IRES-EGFP
Puro

Use

Lentiviral

Tags

EGFP, H2B, IRES, PGK, and mOrange2

Promoter

EF1 alpha-human and PGK-mouse

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Article

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAEMXT-eLACCO1.1

Plasmid

#167946

Purpose

Fluorescent biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

CD59-eLACCO1.1-GPI

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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iOn-CAG∞RFP

Plasmid

#154014

Purpose

Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab7a

Plasmid

#72903

Purpose

Late endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab7a (RAB7A Human)

Use

Lentiviral

Tags

FKBP, mCherry

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBA409

Plasmid

#85971

Purpose

EF1a-sfGFP-Ubc-Neo

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sfGFP
Neo

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-LRRTM1

Plasmid

#82536

Purpose

Lentiviral vector for expressing HRP-LRRTM1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

LRRTM1 (LRRTM1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTAL7a

Plasmid

#48705

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pQC NLS-CFP IX

Plasmid

#37334

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

NLS CFP

Promoter

CMV

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 3

Plasmid

#134345

Purpose

Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 3

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab11a

Plasmid

#72902

Purpose

Recycling endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab11a (RAB11A Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-216

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay sHRPa-KDEL

Plasmid

#73149

Purpose

Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-KDEL

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pcDNA3

Plasmid

#51992

Purpose

mammalian expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only