We narrowed to 6,366 results for: wus

1. pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

   Plasmid

   #177767

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R9A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

   Plasmid

   #177768

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S95A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

   Plasmid

   #177769

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K116A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

   Plasmid

   #177770

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T139A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

   Plasmid

   #177771

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K370A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

   Plasmid

   #177772

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S374A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

   Plasmid

   #177773

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K592A

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

   Plasmid

   #180811

   Purpose

   Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BTLA (Y257F, Y282F)

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-His10-BTLAicd (AA190-289)-TwinStrep

   Plasmid

   #180805

   Purpose

   Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLAint (AA190-289)-TwinStrep (BTLA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pClgn-Lrrc23-FLAG

    Plasmid

    #179921

    Purpose

    Vector backbone. pClgn-promoter driven expression.

    Depositor

    Masahito Ikawa

    Article

    Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Lrrc23 (Lrrc23 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Stop codon removed

    Promoter

    Calmegin

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pClgn-Lrrc23-1D4

    Plasmid

    #179922

    Purpose

    Vector backbone. pClgn-promoter driven expression.

    Depositor

    Masahito Ikawa

    Article

    Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    Lrrc23 (Lrrc23 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Stop codon removed

    Promoter

    Calmegin

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p23-NES-RanCas13b-msfGFP-NES-Flag

    Plasmid

    #165073

    Purpose

    overexpression of RanCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RanCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIG1b_nsp7 (SARS-CoV-2)

    Plasmid

    #169178

    Purpose

    Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    untagged

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIG1c_nsp8 (SARS-CoV-2)

    Plasmid

    #169179

    Purpose

    Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    untagged

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ265C

    Plasmid

    #164715

    Purpose

    Gateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    hAID-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible haid-cbe_v01 entry clo…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ265E1

    Plasmid

    #164718

    Purpose

    Gateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible a3a-cbe_v01 entry clone

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ265E3

    Plasmid

    #164720

    Purpose

    Gateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    eA3A-zCas9(D10A)-UGI
    

    Use

    CRISPR; Gateway compatible ea3a-cbe_v01 entry clo…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T7pr-His6-MBP-TEV-Cas9-BirA*

    Plasmid

    #159993

    Purpose

    Bacterial expression of Cas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    Cas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiV_Neo_ZFP64_Delta_TAD

    Plasmid

    #118698

    Purpose

    Express ZFP64 without transcription activation domain(TAD)

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    ZFP64_Delta_TAD (ZFP64 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG was fused to the N terminal of ZFP64 cDNA

    Expression

    Mutation

    Delete amino acid from 453 to 681

    Promoter

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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