167,601 results

1. pLJM1-Tmem192-mRFP-3xHA

   Plasmid

   #134631

   Purpose

   bait for affinity-isolation of lysosomes

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3xHA, TEV, and mRFP

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF Synaptophysin-mRuby

   Plasmid

   #188980

   Purpose

   Synaptic buton marker

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Rat, Synthetic)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-MBP-2xNLS-tdTomato

   Plasmid

   #104054

   Purpose

   An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-tdTomato
   

   Use

   AAV

   Tags

   NLS

   Promoter

   MBP

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-Flp

   Plasmid

   #122969

   Purpose

   Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

   Depositor

   Lydia Freddolino

   Article

   Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

   Insert

   Flp recombinase
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Aug. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. LentiV_Cas9_Blast

   Plasmid

   #125592

   Purpose

   Lentiviral expression of spCas9 with blasticidin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.GfaABC1D.PI.Lck-GFP.SV40

   Plasmid

   #105598

   Purpose

   AAV expression of membrane bound EGFP from GfaABC1D promoter

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al J Gen Physiol. 2013 Apr 15.

   Has Service

   AAV5

   Insert

   Lck-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_AncBE4max

   Plasmid

   #112094

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-tdTomato (AAV PHP.S)

   Viral Prep

   #28306-PHP.S

   Purpose

   Ready-to-use AAV PHP.S particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV were produced with the PHP.S serotype, which permits efficient transduction of the peripheral nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (Cre-dependent)

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pDRF1-GW ymNeongreen-T2A-mCherry

   Plasmid

   #125700

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mCherry
   

   Expression

   Yeast

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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