We narrowed to 15,156 results for: pET

1. pVRC8400-SARS-CoV-2-NTD-E154K-AVI

   Plasmid

   #176433

   Purpose

   Expresses SARS-CoV-2 NTD domain containing E154K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-NTD-E154K (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   E154K

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVRC8400-SARS-CoV-2-S2P-B.1.429-AVI

   Plasmid

   #176328

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.429 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.429 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   W152C, L452R, D614G

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVRC8400-SARS-CoV-2-S2P-B.1.526-S477N-AVI

   Plasmid

   #176329

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.429 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T95I, D253G, S477N, D614G, A701V

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVRC8400-SARS-CoV-2-S2P-B.1.526-E484K-AVI

   Plasmid

   #176330

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.526 variant containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.429 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T95I, D253G, E484K, D614G, A701V

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVRC8400-SARS-CoV-2-S2P-B.1.617.1-AVI

   Plasmid

   #176333

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.617.1 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q…

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVRC8400-SARS-CoV-2-S2P-D614G-AVI

   Plasmid

   #176324

   Purpose

   Expresses SARS-CoV-2 S2P protein containing D614G mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-D614G (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Desmoplakin II donor only control (FL-based no force control)

   Plasmid

   #118722

   Purpose

   The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-FL-mCherry(Y72L)

   Expression

   Mammalian

   Mutation

   truncation after aa1353; Y72L mutation in mCherry…

   Promoter

   CMV

   Available Since

   Dec. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Desmoplakin II acceptor only control (F40-based no force control)

   Plasmid

   #118720

   Purpose

   The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-F40-mCherry(Y72L)

   Expression

   Mammalian

   Mutation

   truncation after aa1353; Y67G mutation in YPet(sh…

   Promoter

   CMV

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PUF Assembly Kit

   Plasmid Kit

   #1000000051

   Purpose

   Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.

   Depositor

   Huimin Zhao

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223321

    Purpose

    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Inserts

    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVI

    Plasmid

    #181704

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.1.529 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVI

    Plasmid

    #182930

    Purpose

    Expresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD(aa324-526)-B.1.1.529 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVRC8400-SARS-CoV-2-RBD(aa335-526)-B.1.1.529-AVI

    Plasmid

    #181701

    Purpose

    Expresses SARS-CoV-2 RBD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-B.1.1.529 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVRC8400-SARS-CoV-2-NTD-B.1.1.529-AVI

    Plasmid

    #181698

    Purpose

    Expresses SARS-CoV-2 NTD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-B.1.1.529 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid

    #118717

    Purpose

    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry(Y72L)

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His-SUMO-Rab10 Q68L

    Plasmid

    #236720

    Purpose

    Expresses Rab10 Q68L with His tag and SUMO cleavage sequence

    Depositor

    Suzanne Pfeffer

    Article

    Claire Y. et al bioRxiv, 2025

    Insert

    Rab10 Q68L (RAB10 Human)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Q68L

    Promoter

    T7

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRC8400-SARS-CoV-2-RBD-XBB-AVI

    Plasmid

    #196619

    Purpose

    Expresses SARS-CoV-2 RBD protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-XBB
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVRC8400-SARS-CoV-2-RBD-BQ.1.1-AVI

    Plasmid

    #196618

    Purpose

    Expresses SARS-CoV-2 RBD protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BQ.1.1
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, R346T, S371F, S373P, S375F, T376A, D405N, …

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRC8400-SARS-CoV-2-RBD-SD1-BA.2-AVI

    Plasmid

    #184534

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-BA.2 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRC8400-SARS-CoV-2-S2P-BA.2-AVI

    Plasmid

    #184531

    Purpose

    Expresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-BA.2 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, G142D, V213G, G339D…

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-L452R-T478K-AVI

    Plasmid

    #176457

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-K417N-L452R-T478K (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    K417N, L452R, T478K

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI

    Plasmid

    #176450

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    K417N, E484K, N501Y

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVRC8400-SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y-AVI

    Plasmid

    #176451

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    K417T, E484K, N501Y

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVRC8400-SARS-CoV-2-S2P-B.1.617-E154K-L452R-E484Q-D614G-P681R-AVI

    Plasmid

    #176331

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing E154K-L452R-E484Q-D614G-P681R mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E154K, L452R, E484Q, D614G, P681R

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RF21

    Bacterial Strain

    #102803

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pVRC8400-SARS-CoV-2-NTD-AVI

    Plasmid

    #160475

    Purpose

    Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

    Insert

    SARS-CoV-2-NTD
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVRC8400-SARS-CoV-2-RBD-AVI

    Plasmid

    #160476

    Purpose

    Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

    Insert

    SARS-CoV-2-RBD
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pVRC8400-SARS-CoV-2-S2P-AVI

    Plasmid

    #160474

    Purpose

    Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

    Insert

    SARS-CoV-2-S2P
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVI

    Plasmid

    #198466

    Purpose

    Expresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-XBB.1.5 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVI

    Plasmid

    #181695

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.1.529 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge