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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 4341 - 4360 of 14067 results

  1. pcDNA3-InPTapp-Kras

    Plasmid
    #235999
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane.
    Depositor
    Jin Zhang
    Publication
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InPTapp-Kras (PLEKHA1 Human, Synthetic)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235999-plasmid-map-sequence-id-473745

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  2. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid
    #223079
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223079-plasmid-map-sequence-id-447658

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  3. p3E_he1a:ECFP

    Plasmid
    #113880
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    ECFP
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    113880-plasmid-map-sequence-id-222569

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  6. TB202

    Bacterial strain
    #230032
    Purpose
    Fluorescently labelled (mCerulean) E. coli
    Depositor
    Tobias Bergmiller, Calin Guet
    Bacterial resistance
    None
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only

  9. pJY3948

    Plasmid
    #113338
    Purpose
    6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression
    Depositor
    Suzanne Sandmeyer
    Publication
    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.
    Insert
    2-pyrone synthase, codon optimized for Y. lipolytica
    Tags
    6XHis
    Expression
    Yeast
    Mutation
    Please see depositor comments
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113338-plasmid-map-sequence-id-218737

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  12. RAB3B (3DZ8)

    Plasmid
    #32879
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB3B (RAB3B Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 18-190 only
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. PANK3

    Plasmid
    #32872
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK3 (PANK3 Human)
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 10-370 only
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. Kaede-Nucleus-9

    Plasmid
    #57319
    Purpose
    Localization: Nucleus, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    Kaede-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. SETD3

    Plasmid
    #32873
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD3 (SETD3 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4341 - 4360 of 14067 results