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pcDNA5FRT/Flag-FLJ10081

Plasmid

#64775

Purpose

Mammalian expression of Flag-tagged FLJ10081 (NSL3)

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

FLJ10081 (KANSL3 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha

Plasmid

#136434

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX2_P2A_Hygro_Barcode

Plasmid

#120480

Purpose

Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-hPGK-eGFP-SynaptoZip

Plasmid

#122523

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hPGK

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP2A-mNeonGreen

Plasmid

#228525

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

LAMP2 (Lamp2 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7

Plasmid

#221555

Purpose

Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hARdelta120-472

Plasmid

#89088

Purpose

mammalian expression of human androgen receptor with deletion of 120-472 amino acid residues

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

hAR-120-472 N-term deletion (AR Human)

Expression

Mammalian

Mutation

hAR-120-472

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 WT

Plasmid

#74950

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pINDUCER-21-HOXA9

Plasmid

#97041

Purpose

Expresses HOXA9 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

HOXA9 (HOXA9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_TFAP2C_P2A_Hygro_Barcode

Plasmid

#120487

Purpose

Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

TFAP2C (TFAP2C Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF7_P2A_Hygro_Barcode

Plasmid

#120493

Purpose

Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF7 (KLF7 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-mCep290

Plasmid

#27381

Depositor

Joseph Gleeson

Article

Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

Insert

Cep290 (Cep290 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-TdT

Plasmid

#126450

Purpose

Expresses human TdT in mammalian cells. (pTBL206)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROG3_P2A_Hygro_Barcode

Plasmid

#120468

Purpose

Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-FLucStop

Plasmid

#177617

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Mutation

In-frame stop codon added between FLuc and FSE

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K-IRES-Puro

Plasmid

#120570

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HAND2_P2A_Hygro_Barcode

Plasmid

#120447

Purpose

Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HAND2 (HAND2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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