We narrowed to 25,030 results for: Nes

1. pLX302-JSP1-V5 puro

   Plasmid

   #87773

   Purpose

   Expresses V5 tagged human JSP1 (DUSP22)

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   JSP1 (DUSP22 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-sfGO-Matryoshka-LE-LP

   Plasmid

   #100018

   Purpose

   Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   cpsfGFP-LSSmOrange
   

   Use

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Leu and Glu N-term linker and Leu and Pro C-term …

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIMVAd40R

   Plasmid

   #98309

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV P2Gm p53

   Plasmid

   #19751

   Depositor

   Richard Hynes

   Article

   Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

   Insert

   oligo targeting p53 (Trp53 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-T7-hOsa1

   Plasmid

   #17986

   Depositor

   Naoko Tanese

   Article

   Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

   Insert

   Osa1 (ARID1A Human)
   

   Use

   Tags

   T7 epitope

   Expression

   Mammalian

   Mutation

   Missing 5' end

   Promoter

   Available Since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pRSET a MatryoshCaMP6s

   Plasmid

   #100019

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRE-iRFP670

   Plasmid

   #82696

   Purpose

   Expresses CRE and iRFP670 under two separate PGK promoters.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Insert

   CRE, iRFP670
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

   Plasmid

   #178985

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-RTP2-Integration

    Plasmid

    #129459

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP2 (RTP2 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KCC2B_HUMAN_D0

    Plasmid

    #79718

    Purpose

    This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2B
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pSLIK 3xFLAG-KIF20A zeo

    Plasmid

    #192322

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-KIF20A

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KIF20A (KIF20A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

    Plasmid

    #178989

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2xp53_RE

    Plasmid

    #118053

    Purpose

    GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    2 copies of the p53 DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p4xSMAD_RE

    Plasmid

    #118051

    Purpose

    GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    4 copies the SMAD DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK, U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MK14_HUMAN_D0

    Plasmid

    #79680

    Purpose

    This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK14
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV FLIPi P2G_Thy1.1 PTEN

    Plasmid

    #19749

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting PTEN (Pten Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAEL007097-Cas9

    Plasmid

    #100705

    Purpose

    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL007097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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