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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,094 results for: pcdna3.1

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Showing: 4341 - 4360 of 7094 results

  2. pTE4889

    Plasmid
    #88905
    Purpose
    Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Fn crRNA
    hFnCpf1(D917A)
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    88905-plasmid-map-sequence-id-176331

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  3. VBS mutant of alpha catenin sensor

    Plasmid
    #71710
    Purpose
    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-catenin ΔVBS FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 316-405 in α -catenin…
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71710-plasmid-map-sequence-id-142366

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  5. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Publication
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  6. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Publication
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  8. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A and ddRFP B
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pcDNATM3.1/V5-His TOPO-PKCi-K274W

    Plasmid
    #55690
    Purpose
    expresses dominant negative human PKCi tagged with V5
    Depositor
    Pedro Salas
    Publication
    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    A120E and K274W
    Promoter
    CMV
    Available since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 4341 - 4360 of 7094 results