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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 13,928 results for: EGF

Showing: 4361 - 4380 of 13928 results

  3. pMRX-IP-EGFP-LOV2-OMP25

    Plasmid
    #233346
    Purpose
    Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus is deleted and N538E
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233346-plasmid-map-sequence-id-469428

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  4. pMRX-IP-EGFP-LOV2-TMEM106B

    Plasmid
    #233347
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-TMEM106B in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus is deleted (TMEM106B 90-274) and N538E
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233347-plasmid-map-sequence-id-469408

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  5. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid
    #228939
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228939-plasmid-map-sequence-id-469416

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  13. pML624_RAB1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227906
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    227906-plasmid-map-sequence-id-458972

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  15. pCAGGS-MTS(S17A)-EGFP-V5

    Plasmid
    #200928
    Purpose
    expresses MTS(S17A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S17A in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200928-plasmid-map-sequence-id-409553

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  16. pCAGGS-MTS(S23A)-EGFP-V5

    Plasmid
    #200929
    Purpose
    expresses MTS(S23A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    MTS amino acid 23 mutated to Alanine
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200929-plasmid-map-sequence-id-410100

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  17. pCAGGS-MTS(S27A)-EGFP-V5

    Plasmid
    #200930
    Purpose
    expresses MTS(S27A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S27A in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200930-plasmid-map-sequence-id-409412

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  18. pCAGGS-MTS(S31A)-EGFP-V5

    Plasmid
    #200931
    Purpose
    expresses MTS(S31A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S31A in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200931-plasmid-map-sequence-id-410101

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  19. pCAGGS-MTS(S34A)-EGFP-V5

    Plasmid
    #200932
    Purpose
    expresses MTS(S34A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S34A in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200932-plasmid-map-sequence-id-409413

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  20. pCAGGS--MTS(S43A)-EGFP-V5

    Plasmid
    #200933
    Purpose
    expresses MTS(S43A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S43A in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200933-plasmid-map-sequence-id-409552

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Showing: 4361 - 4380 of 13928 results