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pGEXNKI-his-GST-3C-LIC

Plasmid

#108709

Purpose

Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-6xHis-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXa3-nano

Plasmid

#73616

Purpose

Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3-nano

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV mouse Vav1 IRES GFP

Plasmid

#107091

Purpose

Mammalian expression retroviral vector

Depositor

Steven Clifford, Martine Roussel

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTU425

Plasmid

#178984

Purpose

pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

TPR-CHAT H585A C627A

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Plasmid

#204288

Purpose

yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Tags

mRuby3

Expression

Yeast

Promoter

pHis3

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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CoV-2-NSP3-c002

Plasmid

#173084

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))

Expression

Bacterial

Mutation

E1084-E1192, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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NO- DSD Sensor 3

Plasmid

#199787

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pREMI-ZA

Plasmid

#183736

Purpose

Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Sh ble

Use

Random insertional gene deletion in komagataella …

Promoter

TEF1 promoter

Available Since

March 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160C

Plasmid

#46

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

expresses aa580-1330

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
YIp128-CUP1-NUbo-GFP-His6

Plasmid

#212790

Purpose

Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-GFP-His6

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Article

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Tags

mCitrine

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-3C-LIC-kan

Plasmid

#108703

Purpose

Expression of an His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-Tmx4-V5

Plasmid

#120236

Purpose

Expresses V5-tagged Tmx4 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-his-3C-LIC-strep

Plasmid

#108705

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-HOXB3-FLAG

Plasmid

#74508

Purpose

Retroviral expression of HOXB3

Depositor

Lars Rönnstrand

Article

Lindblad et al Biochem Biophys Res Commun. 2015 Nov 27;

Insert

HOXB3 (HOXB3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

unknown

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
ScFv-2ERT2-V

Plasmid

#120553

Purpose

3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

scFvGCN4, sfGFP, GB1, ERT2, VP64

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG eGFP-Oct4

Plasmid

#203789

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou5f1 (Pou5f1 Mouse)

Tags

mEGFP

Expression

Mammalian

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEXNKI-GST-3C-LIC

Plasmid

#108710

Purpose

Expression of a GST-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-nirButterfly

Plasmid

#136591

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1-2xStrep

Plasmid

#138436

Purpose

Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Tags

Strep

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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