We narrowed to 9,286 results for: Pol;

1. pEx-insertion-tet

   Plasmid

   #172211

   Purpose

   For genetic insertion in tetracyclin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Genetic insertion in prevotella copri

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-PB-PGK-tetR-KRAB-2A-Puro

   Plasmid

   #129536

   Purpose

   PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   PB-PGK-tetR-KRAB-2A-Puro
   

   Use

   Unspecified

   Promoter

   hPGK promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-PT2A-GFP-iRFP670-Tdtomato

   Plasmid

   #111813

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   iRFP670
   Tdtomato
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-PT2A-iRFP670-GFP-Tdtomato

   Plasmid

   #111816

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   GFP
   Tdtomato
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pREF-GFP kan

   Plasmid

   #122061

   Purpose

   reporter plasmid for termination and terminator override to express GFP. Marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Inserts

   intergenic terminator from yeast IMD2
   green fluorescent protein
   

   Expression

   Yeast

   Promoter

   GAL1 and GAL1 comes in pYES2

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-Muc2D1D2D3

   Plasmid

   #155215

   Purpose

   Expresses human MUC2 residues 21-1259

   Depositor

   Deborah Fass

   Article

   Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

   Insert

   Muc2 D1D2D3 (MUC2 Human)
   

   Tags

   6xHistadine

   Expression

   Mammalian

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21(+)-His6-Ubc13

   Plasmid

   #212715

   Purpose

   Bacterial expression of His6-Ubc13

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc13
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p426 25Q GAL

   Plasmid

   #1187

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

9. NylB_pET29b

   Plasmid

   #215414

   Purpose

   Expression construct for NylB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB
   

   Expression

   Bacterial

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Promoter

    Vhb

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Expression

    Bacterial

    Promoter

    Vhb

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p426 72Q GPD

    Plasmid

    #1183

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 72Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

    Plasmid

    #172225

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-mCherry-Otx2shRNA1

    Plasmid

    #73979

    Purpose

    Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Myc tag S16-A PTB

    Plasmid

    #23025

    Depositor

    Douglas Black

    Article

    Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

    Insert

    PTB1 (PTBP1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed Serine 16 to Alanine

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

19. CAG-EnR

    Plasmid

    #19715

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    engrailed (en Fly)
    

    Expression

    Mammalian

    Mutation

    Drosophila engrailed repressor domain

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

20. GFAP-Archon-EGFP

    Plasmid

    #187979

    Purpose

    Expresses the fluorescent voltage indicator Archon under the GFAP promoter

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Archon1-EGFP
    

    Use

    AAV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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