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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,007 results for: actin

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Showing: 4361 - 4380 of 6007 results

  1. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

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  13. pAT-9222

    Plasmid
    #124222
    Purpose
    Cloning plasmid for EGxFP assay with self-targeting gRNA-s
    Depositor
    Ervin Welker
    Publication
    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    self-targeting gRNA between EGFP halves
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    124222-plasmid-map-sequence-id-243693

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  15. pCAG-TetR-RNF169UBD

    Plasmid
    #119011
    Purpose
    Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression
    Depositor
    Ralf Kuehn
    Publication
    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TetR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    119011-plasmid-map-sequence-id-233225

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  16. pU6Rosa-CAG-Gal4-Rad18UBD

    Plasmid
    #119012
    Purpose
    Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression
    Depositor
    Ralf Kuehn
    Publication
    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gal4 (GAL4 Budding Yeast)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    119012-plasmid-map-sequence-id-233072

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  19. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid
    #133790
    Purpose
    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized NmeCas9
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CAG
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133790-plasmid-map-sequence-id-268572

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  20. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid
    #136656
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136656-plasmid-map-sequence-id-275491

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Showing: 4361 - 4380 of 6007 results