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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4361 - 4380 of 6962 results

  2. CARMEN

    Plasmid
    #240464
    Purpose
    Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER
    Depositor
    Roland Malli
    Publication
    Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CARMEN
    Expression
    Mammalian
    Available since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240464-plasmid-map-sequence-id-482332

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  3. Actin Binding Domain Tension Sensor

    Plasmid
    #215368
    Purpose
    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.
    Depositor
    Brenton Hoffman
    Publication
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Actin Binding Domain Tension Sensor
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215368-plasmid-map-sequence-id-430713

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  4. Actin Binding Domain Tensionless Control

    Plasmid
    #215371
    Purpose
    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).
    Depositor
    Brenton Hoffman
    Publication
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Actin Binding Domain Tensionless Control
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215371-plasmid-map-sequence-id-430717

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  5. pGR420

    Plasmid
    #213782
    Purpose
    Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank
    Depositor
    Chang Liu
    Insert
    MET15
    Use
    Synthetic Biology
    Expression
    Yeast
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213782-plasmid-map-sequence-id-424626

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  6. pGR481

    Plasmid
    #213783
    Purpose
    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations
    Depositor
    Chang Liu
    Insert
    ura3*
    Use
    Synthetic Biology
    Expression
    Yeast
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213783-plasmid-map-sequence-id-424628

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  7. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Chang Liu
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Expression
    Yeast
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213784-plasmid-map-sequence-id-424627

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  8. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy3 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

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  9. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPLICSFAST-ER-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

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  10. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    ER-SPLICSs-cFAST10
    Tags
    Flag
    Expression
    Mammalian
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210431-plasmid-map-sequence-id-415626

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  15. ER2-mhYFP

    Plasmid
    #186528
    Purpose
    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Monomeric hyperfolder YFP
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186528-plasmid-map-sequence-id-373452

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  20. CoV2-Spike-D614G-Alpha

    Plasmid
    #177961
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)
    Depositor
    Melanie Ott
    Publication
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
    Expression
    Mammalian
    Mutation
    del69, 70, del144, D614G, N501Y, A570D, P681H, T7…
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    177961-plasmid-map-sequence-id-348264

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Showing: 4361 - 4380 of 6962 results