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pGEX-4T-1-Shp2 T7DA/S189D SH2s

Plasmid

#67577

Purpose

Expresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteria

Depositor

Graeme Carnegie

Article

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

Src homology 2 domain-containing phosphatase 2

Tags

GST

Expression

Bacterial

Mutation

Deleted amino acid residues 219-597, changed T73 …

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pRevLCav2deltaC

Plasmid

#60809

Purpose

to make truncation of yeast Rev3

Depositor

Youri Pavlov, Tahir Tahirov

Article

Siebler et al DNA Repair (Amst). 2014 May 9. pii: S156

Insert

REV3 (REV3 Budding Yeast)

Expression

Yeast

Mutation

rev3ΔC encodes for Rev3 lacking the C-terminus (a…

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_MYNN

Plasmid

#45260

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MYNN (MYNN Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN81

Plasmid

#45293

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

deleted 81 N-terminal amino acids

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaC27

Plasmid

#45292

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

deleted 27 c-terminal amino acids

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaN149

Plasmid

#45294

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
pLENTICRISPR-SNRPA-sgRNA1

Plasmid

#165947

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRPA

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRPA (SNRPA Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRPA-sgRNA2

Plasmid

#165948

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRPA

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRPA (SNRPA Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-puro-GFP-3xFLAG

Plasmid

#165951

Purpose

Overexpression of GFP.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

GFP

Use

Lentiviral

Tags

3x-FLAG

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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pWPI-Neo-SLC7A11-HA

Plasmid

#165958

Purpose

Overexpression of SLC7A11.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

HA

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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M51 Super 8x FOPFlash (TOPFlash mutant)

Plasmid

#12457

Purpose

Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

mutant TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

Plasmid

#234826

Purpose

STITCHR R2Tocc editor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

nCas9h840a-xten-R2Tocc

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

ADAR1p150 (ADAR Human)

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSI-Check2-hRluc-NFkB-firefly

Plasmid

#106979

Purpose

Luciferase reporter with internal control for NFkB signal.

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

NFKB recognition sites (NFKB1 Human)

Use

Luciferase

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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CROPseq-iT7

Plasmid

#211699

Purpose

NIS-Seq-compatible lentiviral vector for expressing sgRNAs

Depositor

Jonathan Schmid-Burgk

Article

Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_ENVLPE+

Plasmid

#232427

Purpose

Expression plasmid to produce ENVLPE+ particles

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ENVLPE+

Expression

Mammalian

Promoter

2xCMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_miniENVLPE

Plasmid

#232431

Purpose

Expression plasmid to produce miniENVLPE particles

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

miniENVLPE

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRVdGL-4Cre

Plasmid

#98039

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdGL-4EGFP

Plasmid

#98037

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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DLX2-hygro

Plasmid

#97330

Purpose

Generating lentiviruses to express Dlx2 and hygromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

DLX2 (Dlx2 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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