163,403 results

1. MP6

   Plasmid

   #69669

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. UBQ:RUBY

   Plasmid

   #160909

   Purpose

   RUBY under the control of Ubiquitin promoter/marker for transformation

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and UBIQUITIN promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3Luc-5XNF-kappaB

   Plasmid

   #185695

   Purpose

   Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

   Depositor

   Esther López-Bayghen

   Article

   Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

   Insert

   NF-kappaB (NFKB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   NF-kappaB binding site

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP264

   Plasmid

   #149334

   Purpose

   Bacterial expression of Biotin ligase BirA

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Biotin ligase BirA
   

   Use

   Tags

   His14 and bdNEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7-VEE-IRES-Puro

   Plasmid

   #58970

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZE27GFP

   Plasmid

   #75452

   Purpose

   Plasmid for constitutive expression of gfp

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placIq promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV9)

   Viral Prep

   #112005-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Promoter

   Syn

   Tags

   mRuby3

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pLminP_Luc2P_RE1

   Plasmid

   #90335

   Purpose

   NFkB - p50/p65 pathway gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFKB

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV PHP.eB)

    Viral Prep

    #44362-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only