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Plasmid#112041PurposeCEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URADepositorInsertERG5
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WW-PolyA
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1660
Plasmid#112034PurposeC-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEUDepositorInsertIAA17
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterNoneAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-T2A-LSS-SGFP2
Plasmid#112946PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmScarlet-I and LSS-SGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH
Plasmid#112212PurposeTargeted DNA methylationDepositorInsertN-terminus of dCas9 and DNMT3A (DNMT3A Human)
UseAAVTagsExpressionMutationH840A,N863APromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSS-SGFP2-N1
Plasmid#112938PurposeVisualization of F-actinDepositorInsertLifeAct
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-NM
Plasmid#1127DepositorInsertNM (SUP35 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FRB-myc-RagC
Plasmid#112741Purposeexpress FRB-myc-RagCDepositorInsertFRB-myc-RagC (RRAGC Human)
UseLentiviralTagsFRB, mycExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box
Plasmid#112257PurposeExpresses RGS component of opto-RGS2 in mammalian cellsDepositorInsertCRY2-mCherry-(GGGGS)3-hRGS2box
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIC-LexAGAD
Plasmid#112807Purposegateway cloning destination vector to express enhancer driven LexAGAD in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP2HA
Plasmid#112598PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusionsDepositorTypeEmpty backboneUseTags8His-Avi tagExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CvcE in pGEX4t
Plasmid#112977PurposeFor protein expression and purification of Drosophila CvcEDepositorInsertcycE (CycE Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFE3-donor
Plasmid#112377PurposeCRISPR donor plasmid to tag human transcription factor TFE3 with GFPDepositorInsertTFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
UseCRISPRTagsExpressionMutationhg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGMV-gSWEET4
Plasmid#112796PurposeUniversal BeYDV-derived vector with gRNAs targeting the VviSWEET4 geneDepositorInsertgRNA pair targeting VviSWEET4
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1b
Plasmid#112222Purposemammalian expression of flag tagged GREB1bDepositorInsertGREB1b (GREB1 Human)
UseTags3x flagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/FFT-LL5BIP
Plasmid#112830Purposetransient/stable overexpressionDepositorInsertLL5BIP (2410002F23Rik Mouse)
UseTags2xFlag-TEVExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP1
Plasmid#112595PurposeUsed for expression of protein in E. coli as non-fusionsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertrmtB (RmtB Escherichia coli)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only