We narrowed to 12,331 results for: 112

1. pCR-Blunt_hNANOG

   Plasmid

   #112639

   Purpose

   Plasmid for PCR amplification of hNANOG DNA template for in vitro transcription

   Depositor

   Ganna Bilousova

   Article

   McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

   Insert

   hNANOG (NANOG Human)
   

   Use

   Pcr template

   Promoter

   T7

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOP2S

   Plasmid

   #112597

   Purpose

   Used for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusions

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   Strep tag

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOP5GT

   Plasmid

   #112611

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8His-GST and Avi

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ITPKA_3xmTurquoise

   Plasmid

   #112961

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   ITPKA
   

   Tags

   3x mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOP5MP

   Plasmid

   #112612

   Purpose

   Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8His-MBP and Avi

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1 FRB-myc-RagA

   Plasmid

   #112740

   Purpose

   express FRB-myc-RagA

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   FRB-myc-RagA (RRAGA Human)
   

   Use

   Lentiviral

   Tags

   FRB, myc

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-2HMG-mCherry

   Plasmid

   #112739

   Purpose

   Express 2HMG-mCherry in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al RSC Adv, 2016, 6, 46291-46298

   Insert

   2HMG-mCherry
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV Gsk3 sgRNA/GFP

   Plasmid

   #112733

   Purpose

   Gsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.

   Depositor

   Martin Beaulieu

   Article

   Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

   Insert

   GFP
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJW1665

   Plasmid

   #112039

   Purpose

   Integrating plasmid for expression of ER localized mCherry at TRP1 locus

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   ER-mCherry
   

   Expression

   Yeast

   Promoter

   TPI

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pERF.1.0-gDNA

    Plasmid

    #112395

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF

    Depositor

    Kevin White

    Insert

    ERF (ERF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pERF-donor

    Plasmid

    #112299

    Purpose

    CRISPR donor plasmid to tag human transcription factor ERF with GFP

    Depositor

    Kevin White

    Insert

    ERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LPA2R-P2A-mCherry

    Plasmid

    #112932

    Purpose

    Plasmid co-producing the LPA2 receptor and a mCherry

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    LPA2 receptor
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAT5

    Plasmid

    #112586

    Purpose

    Used for expression of protein in E. coli as non-cleavable C-terminal 6His-tag fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 Flag RagB

    Plasmid

    #112755

    Purpose

    express Flag-RagB

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagB (RRAGB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pExp-DsbA(SS)

    Plasmid

    #112563

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-DsbA(SS) and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAT

    Plasmid

    #112582

    Purpose

    Used for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusions

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-APIGH

    Plasmid

    #112804

    Purpose

    gateway cloning destination vector to express enhancer driven Gal4 in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Han et al Neuron. 2012 Jan 12;73(1):64-78. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7-EF-EYFP-YAP1_WW-PolyA

    Plasmid

    #112290

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDA

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Human YAP1-2gamma with mutations in the WW domain…

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSopB2xHA

    Plasmid

    #112513

    Purpose

    PsopB driven sopB-2xHA and sigE

    Depositor

    Olivia Steele-Mortimer

    Article

    Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

    Insert

    sopB-2xHA/sigE operon
    

    Tags

    2xHA

    Expression

    Bacterial

    Mutation

    SopB C-terminal 2xHA

    Promoter

    PsopB

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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