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Showing: 421 - 440 of 825 results

  1. pCS2+ H2B-mRuby2

    Plasmid
    #99270
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mRuby2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Insert
    mRuby2-3XHA
    Use
    Tags
    H2B
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    99270-plasmid-map-sequence-id-192969

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  2. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid
    #92306
    Purpose
    Expression of humanized BsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huBsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92306-plasmid-map-sequence-id-181649

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  3. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid
    #92304
    Purpose
    Expression of humanized Mb2Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92304-plasmid-map-sequence-id-181652

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  4. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid
    #92305
    Purpose
    Expression of humanized Mb3Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92305-plasmid-map-sequence-id-181650

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  5. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  6. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid
    #169355
    Purpose
    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169355-plasmid-map-sequence-id-333206

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  7. pAW8-aid-3HA-eDSR-Turg1:kanMX6

    Plasmid
    #169363
    Purpose
    C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169363-plasmid-map-sequence-id-332979

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  8. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

    Plasmid
    #169359
    Purpose
    C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169359-plasmid-map-sequence-id-333203

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  9. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

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  10. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  11. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  12. M3-PAmcherry1

    Plasmid
    #114189
    Purpose
    studying clustering of M3 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Insert
    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    Use
    Tags
    3xHA and PAmCherry
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114189-plasmid-map-sequence-id-224304

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  13. His8:MBP-tev-Gly-eK-Flv

    Plasmid
    #83383
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83383-plasmid-map-sequence-id-173970

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  14. His8:MBP-tev-Trp-eK-Flv

    Plasmid
    #83390
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83390-plasmid-map-sequence-id-175070

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  15. His8:MBP-tev-Asp-eK-Flv

    Plasmid
    #83389
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83389-plasmid-map-sequence-id-175068

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  16. His8:MBP-tev-His-eK-Flv

    Plasmid
    #83388
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83388-plasmid-map-sequence-id-175065

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  17. His8:MBP-tev-Lys-eK-Flv

    Plasmid
    #83387
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83387-plasmid-map-sequence-id-175064

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  18. His8:MBP-tev-Leu-eK-Flv

    Plasmid
    #83386
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83386-plasmid-map-sequence-id-175060

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  19. His8:MBP-tev-Arg-eK-Flv

    Plasmid
    #83385
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83385-plasmid-map-sequence-id-173975

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  20. His8:MBP-tev-Phe-eK-Flv

    Plasmid
    #83384
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83384-plasmid-map-sequence-id-173972

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Showing: 421 - 440 of 825 results