We narrowed to 4,336 results for: PC

1. pEDF-PhdRS

   Plasmid

   #127445

   Purpose

   Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

   Depositor

   Wenshe Liu

   Article

   Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Expression

   Bacterial

   Mutation

   N346A-C348A mutations on PylRS

   Promoter

   araBAD

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GABPA_pet28a

   Plasmid

   #131640

   Purpose

   Bacterial expression of ETS gene GABPA

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   GABPA (GABPA Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLexm-gfp-Pept2

   Plasmid

   #167988

   Purpose

   expresses rat PepT2 with a c terminal cleavable GFP-His tag.

   Depositor

   Simon Newstead

   Article

   Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

   Insert

   PepT2 (Slc15a2 Rat)
   

   Tags

   cleavable GFP-his8

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1MycHis/HIP1/ΔE

   Plasmid

   #31252

   Depositor

   Theodora Ross

   Article

   Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

   Insert

   Huntingtin-interacting protein 1 (HIP1 Human)
   

   Tags

   his and myc

   Expression

   Bacterial

   Mutation

   deleted ENTH domain

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3/HIP1/ΔLD

   Plasmid

   #31253

   Depositor

   Theodora Ross

   Article

   Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

   Insert

   Huntingtin-interacting protein 1 (HIP1 Human)
   

   Expression

   Bacterial

   Mutation

   Deltion of nucleotides 767-1335

   Available Since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

6. ELF2_pet28a

   Plasmid

   #131662

   Purpose

   Bacterial expression of ETS gene ELF2

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF2 (ELF2 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1MycHis/HIP1/ΔT

   Plasmid

   #31251

   Depositor

   Theodora Ross

   Article

   Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

   Insert

   Huntingtin-interacting protein 1 (HIP1 Human)
   

   Tags

   his and myc

   Expression

   Bacterial

   Mutation

   deletion of TALIN region

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pMS4 (Empty insertion vector with SEC)

   Plasmid

   #154837

   Purpose

   Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   SEC
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   sqt-1(e1350)

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-At 14-3-3 phi

   Plasmid

   #44939

   Depositor

   Robert Ferl

   Article

   Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

   Insert

   14-3-3 phi (GF14 PHI Mustard Weed)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pET15b-At 14-3-3 lambda

    Plasmid

    #44942

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 lambda (GRF6 Mustard Weed)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pAAV_FLAG_hSF3B1

    Plasmid

    #176590

    Purpose

    Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

    Depositor

    Manoj Pillai

    Article

    Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

    Insert

    SF3B1 (SF3B1 Human)
    

    Use

    AAV

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-UTX(420-633)-H6

    Plasmid

    #183773

    Purpose

    Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    UTX
    

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAct-GST-H3(K27Q)

    Plasmid

    #182843

    Purpose

    a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    GST-histone H3 fusion
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAct-GST-H3

    Plasmid

    #182842

    Purpose

    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    GST-histone H3 fusion
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1_AOX-FLAG

    Plasmid

    #177984

    Purpose

    All-in-one dox-inducible expression of Alternative Oxidase

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    AOX
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA for expression in human cells

    Promoter

    TRE

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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