Addgene

We narrowed to 2,101 results for: Xpa

Showing: 421 - 440 of 2101 results

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*

Plasmid

#191111

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 to TAG

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2796 (pSpy0C4)

Plasmid

#220280

Purpose

integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMK484 (2mAID-Hygro)

Plasmid

#214396

Purpose

2mAID for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

2mAID-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK454 (dTAG-BSD)

Plasmid

#214384

Purpose

dTAG for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSM045

Plasmid

#217399

Purpose

KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE1

Use

Tags

Expression

Bacterial

Mutation

Promoter

PTrc

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSM046

Plasmid

#217400

Purpose

KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTrc

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS160

Plasmid

#215682

Purpose

Guide only plasmid targeting chrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.0

Plasmid

#208299

Purpose

Expresses ABE-RA2.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.1

Plasmid

#208302

Purpose

Expresses ABE-RA3.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.2

Plasmid

#208303

Purpose

Expresses ABE-RA3.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.2-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

E27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.3

Plasmid

#208304

Purpose

Expresses ABE-RA3.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.3-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.0

Plasmid

#208305

Purpose

Expresses ABE-RA4.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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FB406

Plasmid

#203627

Purpose

Promoter of the TAKA-amylase A gene from A. oryzae, maltose/starch-inducible.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PamyB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB407

Plasmid

#203628

Purpose

Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Pef1A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB408

Plasmid

#203629

Purpose

Promoter of the ubiquitin ligase gene from P. digitatum (PDIG_07760).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

P07760

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB367

Plasmid

#203630

Purpose

Module for the expression of geneticin resistance and Nanoluciferase.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PgpdA:Nluc:Ttub::PtrpC:nptII:Ttub

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB273

Plasmid

#203612

Purpose

Terminator of pyr4 gene from T. reesei.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Tpyr4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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