Addgene

We narrowed to 19,664 results for: ace

Showing: 421 - 440 of 19664 results

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BL21 Rosetta2 ΔrecB

Bacterial Strain

#176582

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-sfGFP

Plasmid

#199171

Purpose

used to express a superfolder GFP in E. coli for comparison to supercharged variants

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

superfolder GFP

Use

Tags

MGHHHHHHGGA

Expression

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher-mi3

Plasmid

#112255

Purpose

Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

SpyCatcher-mi3

Use

Tags

C-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQint

Plasmid

#25819

Depositor

George Church

Article

Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

Insert

qQint

Use

Bacterial gene inactivation

Tags

Expression

Mutation

plasmid to make targeted insertions in Clostridiu…

Promoter

Available since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pET21b-RL015A

Plasmid

#42134

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

sfGFP-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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BL21 Rosetta2 GamS

Bacterial Strain

#176584

Purpose

E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-RL018A

Plasmid

#42137

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCerulean-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL016A

Plasmid

#42135

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mYPet-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL027A

Plasmid

#42133

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCherry-mVenus

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL020A

Plasmid

#42138

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mVenus-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pNoRBS

Plasmid

#218167

Purpose

pET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levels

Depositor

Emily Weinert

Article

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short insert

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)

Plasmid

#205045

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)-L1

Plasmid

#205046

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)-L1

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)-L2

Plasmid

#205047

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)-L2

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-mCherry(-6)-L3

Plasmid

#205048

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

mCherry(-6)-L3

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-L1

Plasmid

#205027

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-L1

Use

Tags

KGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-L2

Plasmid

#205028

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-L2

Use

Tags

KGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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