We narrowed to 3,213 results for: actin

1. I208L

   Plasmid

   #184303

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   B-Thymosin followedby hexahistidine tag

   Expression

   Yeast

   Mutation

   I208L

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. I250L

   Plasmid

   #184306

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   B-Thymosin followedby hexahistidine tag

   Expression

   Yeast

   Mutation

   I250L

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. I248L

   Plasmid

   #184305

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   B-Thymosin followedby hexahistidine tag

   Expression

   Yeast

   Mutation

   I248L

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. I64L

   Plasmid

   #184321

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   Hexahistidine followed by B-thymosin

   Expression

   Yeast

   Mutation

   I64L

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. I192L

   Plasmid

   #184302

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   B-Thymosin followedby hexahistidine tag

   Expression

   Yeast

   Mutation

   I192L

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. I71L

   Plasmid

   #184297

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   B-Thymosin followedby hexahistidine tag

   Expression

   Yeast

   Mutation

   I71L

   Promoter

   AOX1

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFD3-Act5c

   Plasmid

   #130278

   Purpose

   Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   pCFD3-Act5c (Act5C Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-UtrCH

   Plasmid

   #26740

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261)

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PaGFP-UtrCH

   Plasmid

   #26738

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   photoactivatable GFP

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261)

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mRFP-UtrCH

    Plasmid

    #26739

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    monomeric RFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261)

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PKA1248

    Plasmid

    #239369

    Purpose

    S.cerevisiae Sla1-SH3#1-3 (5-413) in pGEX6P1

    Depositor

    Kathryn Ayscough

    Insert

    SLA1 (amino acids 5-413)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PKA1279

    Plasmid

    #239375

    Purpose

    GST-Las17 (300-422) P388A

    Depositor

    Kathryn Ayscough

    Insert

    Las17 (amino acids 300-422)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Proline 388 to Alanine

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PKA1247

    Plasmid

    #239368

    Purpose

    S.cerevisiae Sla1-SH3#1-2 (5-131) in pGEX6P1

    Depositor

    Kathryn Ayscough

    Insert

    SLA1 (5-131)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PKA1317

    Plasmid

    #239371

    Purpose

    S.cerevisiae Sla1-SH3#1-2 (5-131) W108A (mutation preventing binding to 2nd SH3)

    Depositor

    Kathryn Ayscough

    Insert

    SLA1 (5-131)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W108A

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PKA1336

    Plasmid

    #239372

    Purpose

    GST-Las17 (300-422) RR(319,322)AA

    Depositor

    Kathryn Ayscough

    Insert

    Las17 (amino acids 300-422)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Arginines 319,322 both to alanine

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PKA1252

    Plasmid

    #239373

    Purpose

    GST-Sec4 (full length) cloned in BamH1 and Sal1 sites of pGEX-6P1

    Depositor

    Kathryn Ayscough

    Insert

    Sec4 (SEC4 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PKA1284

    Plasmid

    #239367

    Purpose

    GST-Las17 (342-394) minimal component

    Depositor

    Kathryn Ayscough

    Insert

    Las17 (amino acids 342-392)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment corresponding to amino acids 342-392

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-UtrCH

    Plasmid

    #26737

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261).…

    Promoter

    CMV IE94

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PKA1280

    Plasmid

    #239370

    Purpose

    His-Las17 (300-422) pTOPO 6xHis-Las17 (300-422) as a BamH1-HindIII fragment

    Depositor

    Kathryn Ayscough

    Insert

    Las17 (amino acids 300-422)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PKA1337

    Plasmid

    #239376

    Purpose

    GST-Las17 (300-422) RR(319,322)AA, RR(349,350)AA, and RR(382,383)AA

    Depositor

    Kathryn Ayscough

    Insert

    Las17 (amino acids 300-422)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    RR(319,322)AA, RR(349,350)AA, and RR(382,383)AA

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge