Addgene

We narrowed to 881 results for: fkbp

Showing: 421 - 440 of 881 results

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FKBP-beta2(616-951)-mRuby2

Plasmid

#100730

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

FKBP and mRuby2

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-Myc

Plasmid

#177908

Purpose

human CD81-TEV cleavage site-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81 (CD81 Synthetic, Human)

Use

Tags

FKBP, Myc tag, and TEV protease cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

Plasmid

#80004

Purpose

miRFP709 GAF domain fused to FKBP and NLS

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Use

Tags

GAF domain of miRFP709 and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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C1-FKBP(F36V):mCherry-Akt2(K14A)

Plasmid

#72906

Purpose

Recruitable Akt2(K14A) mutant

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

RAC-beta serine/threonine-protein kinase (AKT2 Human)

Use

Tags

FKBP(F36V), mCherry

Expression

Mammalian

Mutation

K14A mutation

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry-FKBP-TIAM1(DHPH)

Plasmid

#176138

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-FKBP-GEF(TIAM1)

Plasmid

#85156

Purpose

acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEntry Tshld(FKBP F36V)-GFP

Plasmid

#53260

Purpose

pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld(FKBP F36V)-GFP

Use

Tags

EGFP, Flag, and HA

Expression

Mutation

FKBP F36V

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

Plasmid

#171429

Purpose

Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C261

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-mcherry-FKBP-TIAM1(DHPH)

Plasmid

#176135

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Tags

Expression

Mutation

See Depositor Comments Below

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD54(L53P,L67P)

Plasmid

#172458

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

changed Leucines 53 and 67 to proline (breaks coi…

Promoter

CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-GTSE1(638-720)-GFP

Plasmid

#100735

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

GTSE1 (GTSE1 Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

C-terminus only

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBsd-P2a-Fkbp-3xF-GGSG

Plasmid

#235514

Purpose

N-term tagging construct: clone into ZraI and AfeI sites

Depositor

Brian Hendrich

Insert

FKBP

Use

Mouse Targeting

Tags

Bsd, FKBP, 3xFLAG

Expression

Mutation

None

Promoter

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGGSG-FKBP-2HA-P2a-Pac

Plasmid

#235521

Purpose

C-term tagging construct: clone into PmlI and AfeI sites

Depositor

Brian Hendrich

Insert

FKBP

Use

Mouse Targeting

Tags

FKBP, 2xHA, Puro

Expression

Mutation

None

Promoter

Available since

May 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGGSG-FKBP-3xF-T2a-Bsd

Plasmid

#235520

Purpose

Cloning vector for GGSG-FKBP-3xFLAG-T2A-Bsd. Clone into ZraI and AfeI

Depositor

Brian Hendrich

Insert

FKBP

Use

Mouse Targeting

Tags

Fkbp, 3xFALG, Bsd

Expression

Mutation

None

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBsd-T2a-Fkbp-3xF-GGSG

Plasmid

#235515

Purpose

N-term tagging construct: clone into ZraI and AfeI sites

Depositor

Brian Hendrich

Insert

FKBP

Use

Mouse Targeting

Tags

Bsd, FKBP, 3xFLAG

Expression

Mutation

None

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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XZ135 CMV-TO-FKBP-ADARdd(QA) (FLP-IN)

Plasmid

#233376

Purpose

FKBP fused with ADAR2dd, with E488Q and T501A double mutation

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET30-10His-FKBP(f36v)-SNAP

Plasmid

#200282

Purpose

For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

FKBP-SNAPtag

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p-TID-FKBP-HA3

Plasmid

#228010

Purpose

Expresses TurboID-FKBP fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-HIS3MX6

Plasmid

#228025

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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