We narrowed to 1,670 results for: sfGFP

1. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Use

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-sfGFP-3xP3-RFP

   Plasmid

   #127566

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-scFvGCN4sfGFP-VP64-GB1

   Plasmid

   #141417

   Purpose

   Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

   Depositor

   Izuho Hatada

   Article

   Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

   Insert

   scFvGCN4sfGFP-VP64-GB1
   

   Use

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-sfGFP-iCre-ERT2

   Plasmid

   #125749

   Purpose

   sfGFP and iCreERT2 fused by unskippable linker

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   sfGFP-iCre-ERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRBC-sfGFP-134/150TAG

   Plasmid

   #174077

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   D134TAG + N150TAG

   Promoter

   T7

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4TO-24xGCN4_v1-P2A-sfGFP

   Plasmid

   #61057

   Purpose

   sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   24xGCN4_v1 and sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pSB1C3-pT3-tetR-sfGFP

   Plasmid

   #140871

   Purpose

   T3 promoter driven synthesis of TetR-sfGFP

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   TetR-sfGFP expressed with T3 promoter
   

   Use

   Synthetic Biology

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCOLA banana sensor sfGFP

   Plasmid

   #107367

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   banana-detecting toehold + sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRF1-GW yosfGFP-T2A-mCherry

   Plasmid

   #118448

   Purpose

   Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   yosfGFP-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_EF1a-CasRx-msfGFP-2A-Blast

    Plasmid

    #226010

    Purpose

    CasRx protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    CasRx RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid

    #212120

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    

    Use

    Tags

    His6 and SUMO-TAG35

    Expression

    Bacterial

    Mutation

    E35TAG (within SUMO encoding sequence)

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

    Plasmid

    #186227

    Purpose

    mRNA synthesis of rapalog-induced cell adhesion molecule

    Depositor

    Yoshihiro Morishita

    Article

    Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

    Insert

    mRNA synthesis of rapalog-induced cell adhesion molecule
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    FRB (T2098L)

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

    Plasmid

    #133572

    Purpose

    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid

    #133577

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid

    #133573

    Purpose

    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HCMVgO-sfGFP (Merlin)

    Plasmid

    #136452

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCOLA kiwi sensor sfGFP

    Plasmid

    #107368

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    kiwi-detecting toehold + sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB CAG-sfGFP-iCre-ERT2

    Plasmid

    #137856

    Purpose

    CAG-sfGFP-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-sfGFP-DogTag Loop B

    Plasmid

    #171780

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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