We narrowed to 986 results for: transfection
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Plasmid#156422PurposeMammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cellsDepositorInsertSARS-CoV-2 3a protein
TagsEGFP-HisExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPCX-Cx43-FS-IRES-GFP
Plasmid#65438PurposeMammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral productionDepositorInsertCx43 (GJA1 Human)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationframeshift (FS) mutation 35delAPromoterCMVAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H2BC11-MMEJ
Plasmid#207760PurposeMMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hiv7CMV-Cremyc-2A-GFP
Plasmid#117148PurposeExpress Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.DepositorInsertCremyc-2A-GFP
UseLentiviralAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-dKARD
Plasmid#114053Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-dKARD (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance): AGATACTAGCT…Available SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Adcy3-3xFlag
Plasmid#245989PurposeExpress 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Gpr45
Plasmid#245982PurposeExpress wild-type mouse GPR45 transiently via transfection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Gpr45-Y287C
Plasmid#245983PurposeExpress mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Gpr45-S214P
Plasmid#245984PurposeExpress mouse GPR45-S214P mutant transiently via transfection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-Gnai1
Plasmid#245996PurposeExpress HA N-terminal tagged mouse Galphai1 transiently via transfection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
FHpHG_mTent5c_LxxAA (L3m)
Plasmid#221441PurposeTransfection into the cells for the reporter or qPCR assay.DepositorInsertTent5c_LXXAA (L3m) (Fam46c Mouse)
TagsFLAG-2xHA-(HRV 3C site)-6xHis-EGFPExpressionMammalianMutationL332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…Available SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H2BC11
Plasmid#227331PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-H2BC11
Plasmid#227330PurposeDonor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-PLK4
Plasmid#227311PurposeDonor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)DepositorInsertPLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-PCNT
Plasmid#227285PurposeDonor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)DepositorInsertPCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-sTagRFP-TUBA1B
Plasmid#207765PurposeDonor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTOPO-HqERV(C1-20)
Plasmid#164090Purposetransfection of Hq-ERV with light-inducible promoterDepositorInsertHq-ERV
UseEukaryotic expression vectorMutation5'-LTR replaced with light-regulatable C1-20…Promoterlight-activatable C1-20Available SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-3A
Plasmid#114054Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-3A (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-S670A…Available SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-3D
Plasmid#114055Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-3D (BUB1B Human)
TagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-S670D…Available SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only