We narrowed to 1,270 results for: vent

1. pTBL3342 PB-PEmax-puro

   Plasmid

   #226674

   Purpose

   PiggyBac cargo vector encoding PEmax and puromycin resistance.

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   PEmax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL3428 MTK234-LacO-BtoApegRNA

   Plasmid

   #226669

   Purpose

   MTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNA

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   hU6-LacOS-BtoApegRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6-LacOS

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL3331 MTK234-TetO-AtoBpegRNA

   Plasmid

   #226668

   Purpose

   MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   hU6-TetO-AtoBpegRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6-TetO

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL3346 PB-nicking guide-blast

   Plasmid

   #226665

   Purpose

   PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   protective nicking sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xMS2 TR knockin sgRNA 1

   Plasmid

   #207607

   Purpose

   sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   cgactcgcccggcagcgcac
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xMS2 TR knockin sgRNA 2

   Plasmid

   #207608

   Purpose

   sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   acccccaaacctgactgact
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TR knockout HRD

   Plasmid

   #207579

   Purpose

   Homologous recombination donor replacing TR with a puro cassette.

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   puro resistance cassette flanked by TR locus sequences excluding TR
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDN0520 (pDEST, TRP1)

   Plasmid

   #210481

   Purpose

   pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Destination vector for gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hy_pRnrS eGFP SV40

   Plasmid

   #195062

   Purpose

   Expresses eGFP mRNA from the Drosophila RnrS promoter

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   RnrS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hy_pAna eGFP SV40

    Plasmid

    #195063

    Purpose

    Expresses eGFP mRNA from the Drosophila Ana promoter

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ana

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET16b-RPA4/His14 (MSW#949)

    Plasmid

    #208075

    Purpose

    Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA4, RPA14(His-tagged)
    

    Use

    Tags

    10xHis tag on RPA14

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN BFP

    Plasmid

    #188644

    Purpose

    This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dcp2C Δ5H-H1

    Plasmid

    #163584

    Purpose

    express Dcp2 FL-H1 at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of aa1-326; the first HLM (EDQLKSYAEEQ…

    Promoter

    DCP2 promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dcp2 300 AAAA

    Plasmid

    #163579

    Purpose

    express Dcp2 300 AAAA at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of C-terminal domain (301-970); mutati…

    Promoter

    DCP2 promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dcp2 300 ΔH1

    Plasmid

    #163578

    Purpose

    express Dcp2 300 ΔH1 at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of C-terminal domain (301-970); Lysine…

    Promoter

    DCP2 promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti_stgRNA-Puro

    Plasmid

    #164124

    Purpose

    Backbone for lentiviral stgRNA library construction (library 1 and 2)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

    Plasmid

    #91802

    Purpose

    Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIVT/Acrosin-GFP-GPI

    Plasmid

    #122141

    Purpose

    For making acrosin-GFP-GPI RNA

    Depositor

    Carmen Williams

    Article

    Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

    Insert

    acrosin-GFP-GPI
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGMCKq20-T38S38-0X

    Plasmid

    #49522

    Purpose

    Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    constitutive TetR38
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptb38

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. spitz (9 kb)

    Plasmid

    #38340

    Depositor

    Norbert Perrimon

    Article

    Rutledge et al Genes Dev. 1992 Aug;6(8):1503-17.

    Insert

    spitz (spi Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only