Addgene

We narrowed to 872 results for: vill

Showing: 421 - 440 of 872 results

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PCDNA3-TADA1-HFD(140-230)

Plasmid

#105984

Purpose

transient express TADA1-HFD fragment in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1-HFD (140-230) (Tada1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCS-flt4um159-HA

Plasmid

#67704

Purpose

Expression construct

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

flt4 (flt4 Zebrafish)

Use

Tags

HA

Expression

Mammalian

Mutation

um159

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCS-Fv2Eflt4um200-2a-tdTomato

Plasmid

#67698

Purpose

Expression construct

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4-2A-tdTomato (flt4 Zebrafish)

Use

Tags

2A-tdTomato and HA

Expression

Mutation

um200

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

Plasmid

#234826

Purpose

STITCHR R2Tocc editor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

nCas9h840a-xten-R2Tocc

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4-NHK-MYC-His6

Plasmid

#185350

Purpose

Assessing of ubiquitination in cells

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

a1-antitrypsin Hong Kong Null mutant

Use

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

NHK mutant

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-p3-PE(1153-dRnH)-C-term

Plasmid

#187182

Purpose

p3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1

Depositor

Gerald Schwank

Article

Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

Insert

PE2 from plasmid #132775 with shortened RnaseH domain

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCTRE-CD4

Plasmid

#114010

Purpose

Dox-inducible Cas9 expression with a CD4 selection marker

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBA439

Plasmid

#85967

Purpose

Perturb-seq vector backbone

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 K720A

Plasmid

#19939

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 K720A (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a NEDD8 modification mutant

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-DNAJC5

Plasmid

#205727

Purpose

Mammalian expression of DNAJC5

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pFLAG AXL

Plasmid

#105933

Purpose

Mammalian expression of Flag-tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUBC-EBFP2

Plasmid

#212314

Purpose

Lentiviral backbone with EBFP2 reporter

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_EGFP

Plasmid

#73836

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

Plasmid

#211904

Purpose

Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

CD19-CAR-28z-2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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sgTrack-BFP

Plasmid

#164273

Purpose

Empty sgRNA expression vector with co-expression of BFP reporter

Depositor

Charles Rudin

Article

Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EFS

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab6 T27N

Plasmid

#46782

Purpose

expression of T27N Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 T27N (RAB6A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T27N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only