We narrowed to 11,501 results for: ENA
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Plasmid#74056Purposeretroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion proteinDepositorInsertBirA biotin ligase
UseRetroviralTagsP2A-mCherryExpressionMammalianPromoterMSCV-LTRsAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22D
Plasmid#91136PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantMutationD10A, H840AAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
C0040 (tetR)_CD
Plasmid#66027PurposeMoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D]DepositorInsertTranscription factor
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-WHaloCaMP1a-EGFP
Plasmid#205314PurposeExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driverDepositorInsertWHaloCaMP1a-EGFP
ExpressionInsectAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-LOV-Turbo-V5
Plasmid#199666Purposeexpresses LOV-Turbo in the mammalian mitochondria, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsCOX4I1 mito targeting sequence and V5ExpressionMammalianPromoterTRE3GAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
TagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-Q61L
Plasmid#12981DepositorInsertRac1 constitutively active (RAC1 Human)
TagsEGFPExpressionMammalianMutationQ61L Constitutively activeAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHeACT-mEGFP-NLS
Plasmid#205003PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV_pSyn_Mito-LOV-Turbo-V5
Plasmid#199669Purposeexpresses Mito-LOV-Turbo-V5 in neurons, AAV vectorDepositorInsertLOV-Turbo
UseAAVTagsCOX4I1 mito targeting sequence and V5PromoterSynapsinAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-Q61L
Plasmid#12986DepositorInsertCdc42 constitutively active (CDC42 Human)
TagsEGFPExpressionMammalianMutationQ61L Constitutively activeAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
lenti-CDDR
Plasmid#149355PurposeCRISPR/Cas9-based dual-fluorescent DSB repair reporterDepositorInsertsCDDR Reporter
Blasticidin resistance
UseLentiviralExpressionMammalianPromoterEF-1a and hPGKAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
Plasmid#136382PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-VPR
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClβ.CreON
Plasmid#196996PurposeCre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClβ -YFP
UseAAV and Cre/LoxTagsYFPPromotershort CAGAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R2
Plasmid#61383PurposeExpresses TRAIL receptor 2DepositorAvailable SinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClα.CreON
Plasmid#196995PurposeCre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClα -Cerulean
UseAAV and Cre/LoxTagsCeruleanPromotershort CAGAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-Myc tag
Plasmid#67617PurposeA pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…TagsC-terminal MycExpressionMammalianPromoterCMV and SP6Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBR1.cas9-NT
Plasmid#197871PurposeConstitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)DepositorInsertstrancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
ExpressionBacterialAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3717
Plasmid#191477PurposePacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFPDepositorAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only