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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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His-GFP SNAP25

Plasmid

#170872

Purpose

Mammalian expression of His-GFP SNAP25.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

SNAP 25 (Snap25 Rat)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pC007 - pRFP constitutive expression

Plasmid

#79156

Purpose

RFP constitutively expressed

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

RFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pArcCreER

Plasmid

#46389

Depositor

Liqun Luo

Article

Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

Inserts

Arc (Arc Mouse)
CreERT2

Use

Mouse Targeting

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-TOPO-mir17-92

Plasmid

#21109

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR-17-92 cluster (MIR17 Human)

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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negMaLionR

Plasmid

#113909

Purpose

negative control for Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/VChR1-EYFP

Plasmid

#20955

Purpose

Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-mCherry

Plasmid

#80946

Purpose

human AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

mCherry

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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mAzurite-16xRhoBAST

Plasmid

#196347

Purpose

Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

mAzurite-16xRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFB1_hMLH1

Plasmid

#129426

Purpose

Expression human MLH1 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MLH1 (MLH1 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2--3.5ubb:plin2-tdtomato

Plasmid

#172534

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR AAA mutant

Plasmid

#13508

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR AAA (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S10

Plasmid

#127829

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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sc AAV miR26a eGFP

Plasmid

#21894

Depositor

Joshua Mendell

Article

Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

Insert

EF1 alpha -miR26a GFP p(A)

Use

AAV; Sc

Tags

GFP

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-TRAF6-wt

Plasmid

#21624

Depositor

John Kyriakis

Article

Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

Insert

TNF receptor-associated factor 6 (Traf6 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Article

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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GST-TACC3-His6

Plasmid

#69106

Purpose

For bacterial expression of recombinant human TACC3 tagged with GST and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Tags

GST and His6

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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