Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,803 results for: alp;

Showing: 4381 - 4400 of 7803 results

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pHAGE-PIK3R1-Q302*

Plasmid

#116601

Purpose

Lentiviral expression of PIK3R1 Q302*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

Q302*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-R348*

Plasmid

#116603

Purpose

Lentiviral expression of PIK3R1 R348*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

R348*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-R386*

Plasmid

#116604

Purpose

Lentiviral expression of PIK3R1 R386*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

R386*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-D464N

Plasmid

#116584

Purpose

Lentiviral expression of PIK3R1 D464N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

D464N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-F398Lfs*6

Plasmid

#116589

Purpose

Lentiviral expression of PIK3R1 F398Lfs*6

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

F398Lfs*6

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-G353R

Plasmid

#116591

Purpose

Lentiviral expression of PIK3R1 G353R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

G353R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-I381Kfs*15

Plasmid

#116595

Purpose

Lentiviral expression of PIK3R1 I381Kfs*15

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

I381Kfs*15

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSN29

Plasmid

#100114

Purpose

expresses a chimeric cMYC-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to full length HP1 alpha (MYC, CBX5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSN17

Plasmid

#100104

Purpose

expresses a chimeric OCT4-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to full length HP1 alpha (POU5F1, CBX5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-Optop38i-dsm

Plasmid

#89748

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i dark-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.W140C

Plasmid

#82784

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Mutation

W140C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.A541G

Plasmid

#82771

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Mutation

A541G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.S143F

Plasmid

#81440

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Mutation

S143F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Btk(PH)Akt1(KD)

Plasmid

#86591

Purpose

Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)-KD(Akt1) fusion (AKT1, BTK Human)

Tags

10xHis

Expression

Insect

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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CAMK2A gRNA (BRDN0001162457)

Plasmid

#76930

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2A (CAMK2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAK1 gRNA (BRDN0001147867)

Plasmid

#76465

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK1 (PAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAK1 gRNA (BRDN0001145359)

Plasmid

#76466

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK1 (PAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-M85E-3XHA

Plasmid

#242416

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a M85E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D M85E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-V82E M85E M92E-3XHA

Plasmid

#242418

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with V82E, M85E, and M92E mutations and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D V82E M85E M92E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D,F5D-V82E-3XHA

Plasmid

#242393

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a V82E mutation and a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D V82E

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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