We narrowed to 8,355 results for: construct

1. MK03_HUMAN_D0

   Plasmid

   #79688

   Purpose

   This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK03
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. PAK4_HUMAN_D0

   Plasmid

   #79698

   Purpose

   This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PAK4 (PAK4 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. PAK6_HUMAN_D0

   Plasmid

   #79703

   Purpose

   This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PAK6 (PAK6 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pGWB425

   Plasmid

   #74819

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMV-TRP1

   Plasmid

   #65335

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   TRP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC57kan-T7-gRNA-U6 V2

   Plasmid

   #115520

   Purpose

   To construct multiple sgRNA expression vector for CRISPR

   Depositor

   Xingxu Huang

   Insert

   sgRNA scaffolf-U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNP160

   Plasmid

   #106362

   Purpose

   To insert a single copy of a construct at a site on Chr II, corresponding to RDE-1 expressed specifically in the intestine, for tissue-specific RNAi.

   Depositor

   Jonathan Ewbank

   Insert

   ttTi5605_SEC_mtl-2p_RDE-1_3'rde-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-Pur-mEGFP

   Plasmid

   #186329

   Purpose

   Fluorescent reporter system

   Depositor

   Bertrand Collet

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIVTR(T7A)-Cas9-T2A-mClover3

   Plasmid

   #172293

   Purpose

   In vitro transcription of Cas9-T2A-mClover3 synthetic construct

   Depositor

   Patrik Verstreken

   Insert

   Cas9-T2A-mClover3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter A-initiating

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIVTR(T7A)-PuroR-T2A-mScarlet

    Plasmid

    #172301

    Purpose

    In vitro transcription of PuroR-T2A-mScarlet synthetic construct

    Depositor

    Patrik Verstreken

    Insert

    PuroR-T2A-mScarlet
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBSVSG221KOblast

    Plasmid

    #59733

    Purpose

    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.

    Depositor

    Gloria Rudenko

    Insert

    VSG221 knockout construct
    

    Use

    For homologous recombination into vsg221 expressi…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGMC00018 (aka pRC0082)

    Plasmid

    #195320

    Purpose

    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB CAG Tir1 iHyg

    Plasmid

    #235506

    Purpose

    expression construct for OsTir1, Hyg resistance

    Depositor

    Brian Hendrich

    Insert

    OsTir1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    None

    Promoter

    CAG

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB CAG Tir1 Bsd

    Plasmid

    #235505

    Purpose

    expression construct for OsTir1, Bsd resistance

    Depositor

    Brian Hendrich

    Insert

    OsTir1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    None

    Promoter

    CAG

    Available since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNP148

    Plasmid

    #106358

    Purpose

    To insert a single copy of a construct at a site on Chr II, corresponding to a gain-of-function allele of gpa-12 expressed specifically in the adult epidermis.

    Depositor

    Jonathan Ewbank

    Insert

    col-62p_gpa-12gf_3'unc-54
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Orf3a-mCherry

    Plasmid

    #165138

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Orf3a (ORF3a codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Nsp12-EGFP

    Plasmid

    #165116

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp12 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nsp8-EGFP

    Plasmid

    #165113

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp8 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nsp7-mCherry

    Plasmid

    #165134

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-R-GECO1

    Plasmid

    #32444

    Purpose

    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge