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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 4421 - 4440 of 16276 results

  2. CAPN1

    Plasmid
    #25607
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN1 (CAPN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 29-360
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. CBX2

    Plasmid
    #25158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX2_04 (CBX2 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    25158-plasmid-map-sequence-id-472908

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  5. pSUB11

    Plasmid
    #169699
    Purpose
    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Publication
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    3xFLAG tag
    Expression
    Bacterial
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169699-plasmid-map-sequence-id-336760

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  7. pMA-SpCas9-g7

    Plasmid
    #80790
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80790-plasmid-map-sequence-id-161640

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  8. pMA-SpCas9-g6

    Plasmid
    #80789
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80789-plasmid-map-sequence-id-161638

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  10. MLM3713

    Plasmid
    #49946
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x FLAG Tet1CD RH-4 (RHOXF2 Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  11. pJFA345D4

    Plasmid
    #49238
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
    Use
    TALEN; Tale-tet1 expression vector
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    49238-plasmid-map-sequence-id-72805

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  20. G protein alpha-s-CFP

    Plasmid
    #55793
    Purpose
    G protein alpha-s internally tagged with ECFP and EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-alpha-s-EE-ECFP (Gnas Rat)
    Tags
    ECFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    His was substituted for Asn164 in ECFP (Clontech)…
    Promoter
    CMV
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    55793-plasmid-map-sequence-id-86730

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Showing: 4421 - 4440 of 16276 results