Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,352 results for: Cas9

Clear keywords
Showing: 4441 - 4460 of 14352 results

  6. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid
    #217801
    Purpose
    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217801-plasmid-map-sequence-id-432878

    Enlarge

  7. AAVS1-iPE-Plus

    Plasmid
    #214062
    Purpose
    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214062-plasmid-map-sequence-id-429371

    Enlarge

  8. pCMV-PE-Plus

    Plasmid
    #214079
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214079-plasmid-map-sequence-id-429365

    Enlarge

  9. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Publication
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132393-plasmid-map-sequence-id-341619

    Enlarge

  13. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid
    #172841
    Purpose
    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172841-plasmid-map-sequence-id-341031

    Enlarge

  14. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid
    #112922
    Purpose
    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker
    Depositor
    Kosuke Yusa
    Publication
    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    Use
    Gateway Cloning
    Expression
    Mammalian
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    112922-plasmid-map-sequence-id-217731

    Enlarge

  17. pJM149

    Plasmid
    #134168
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Publication
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC E118A R129A, E. coli codon optimized, CRISPR…
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    134168-plasmid-map-sequence-id-271754

    Enlarge

Showing: 4441 - 4460 of 14352 results