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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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LentiGuide_DKO

Plasmid

#183193

Purpose

Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

Depositor

Lang Li

Article

Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6, mU6

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO MYC3

Plasmid

#177848

Purpose

Bacterial Expression of MYC3

Depositor

Karsten Melcher

Article

Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

Insert

MYC3 (MYC3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Tags

mRuby2

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pM162

Plasmid

#173221

Purpose

Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Inserts

ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
MCP-mCherry-GFP11-GB1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Tags

HaloTag

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-5X-1-LARP7

Plasmid

#113545

Purpose

Produces an N-terminal GST-tagged full length LARP7 protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

LARP7 (LARP7 Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.hSynap.(cyto).iGlucoSnFR2

Plasmid

#244091

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRK172-α-syn-TEV-GFP

Plasmid

#166671

Purpose

Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

Depositor

Iris Lindberg

Article

Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

Insert

mouse synuclein (from V. Lee) (Snca Mouse)

Tags

GFP

Expression

Bacterial

Mutation

added a TEV cleavage site sequence between synucl…

Promoter

CMV

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TAP DHX36

Plasmid

#53547

Purpose

mammalian expression of DHX36

Depositor

Kathleen Collins

Article

Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

Insert

DHX36 (DHX36 Human)

Tags

TAP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCVFLAG-ZFP423

Plasmid

#24764

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

Insert

Zinc Finger Protein 423 (Zfp423 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-Full-Length-MePCE

Plasmid

#113539

Purpose

Produces an N-terminal GST-tagged full length MePCE protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2

Plasmid

#244110

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

Plasmid

#218860

Purpose

10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCG-ICAM(1235)-nrxn1b

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1

Plasmid

#70145

Purpose

mammalian expression of MKLP1 with an eGFP fusion

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pETM33_IRF3_SMAD/FHA

Plasmid

#178479

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

IRF3_IRF3 (IRF3 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR_DKO

Plasmid

#183192

Purpose

Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

Depositor

Lang Li

Article

Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6, mU6

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-SNAP

Plasmid

#175295

Purpose

Track mature FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with SNAP-tag

Tags

SNAP-tag

Expression

Mammalian

Available Since

Oct. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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