We narrowed to 2,346 results for: puc19

1. pMB72

   Plasmid

   #73088

   Purpose

   C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

   Depositor

   Mike Boxem

   Article

   Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

   Insert

   GFP-Avi tag
   

   Use

   Tags

   GFP and Avi

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. UPRT-mCh-Nluc-P2A-neo-UPRT

   Plasmid

   #135015

   Purpose

   Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   mCh-Nluc-P2A-neo
   UPRT 5'UTR
   UPRT 3'UTR
   

   Use

   Cryptosporidium expression

   Tags

   mCherry and Nluc-neo

   Expression

   Mutation

   Promoter

   Cryptosporidium actin and enolase

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LADL Bridge + Target Zfp462-Klf4SE

   Plasmid

   #127668

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and hU6

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAP1892-1

   Plasmid

   #105247

   Purpose

   human GFP11 with tagRFP 33bp downstream in Lamin A/C

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LADL Bridge + Promoter Only Target

   Plasmid

   #127669

   Purpose

   Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   CRY2-HA-2A-mCherry and gRNAs 115 and 117
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and hU6

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDV12 [psng-1::SNG-1::CRY2olig(535)]

   Plasmid

   #197596

   Purpose

   Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   E490G

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRHD-TetR-GAL80-Rho1-GAL4

   Plasmid

   #218274

   Purpose

   Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

   Depositor

   Christopher Howard

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   DHFR (P66L), Rho1 (C17R)

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRGAL43C

   Plasmid

   #218278

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRHDGAL3C

    Plasmid

    #218276

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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