We narrowed to 5,259 results for: PID

1. LCV2_mClover3

   Plasmid

   #155095

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIP mScarlet-MOSPD2 RD/LD

   Plasmid

   #186476

   Purpose

   Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIP mScarlet-ER [TM(SAC1)]

   Plasmid

   #186572

   Purpose

   Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   SAC1 (SACM1L Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-terminal transmembrane region of the protein: 5…

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK2657

   Plasmid

   #219741

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   hispidin- synthase from Mycena citricolor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. H516 SONIC KrasG12D-IRES-luciferase donor

   Plasmid

   #138178

   Purpose

   CRISPR SONIC: Kras G12D luciferase donor plasmid.

   Depositor

   Wen Xue

   Article

   Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Nhej donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PuroR-P2A-DD-TCOF1-donor

   Plasmid

   #60747

   Purpose

   Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pMXs-IRES-blast GFP-SPNS1

   Plasmid

   #198571

   Purpose

   SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BlastR-P2A-DD-TCOF1-donor

   Plasmid

   #60748

   Purpose

   Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pJFRC7-JEDI-2P

   Plasmid

   #179461

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-DNER

    Plasmid

    #51053

    Purpose

    expression of HA-DNER in mammalian cells

    Depositor

    Mineko Kengaku

    Article

    Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

    Insert

    DNER (Dner Mouse)
    

    Use

    Tags

    HA and signal peptide

    Expression

    Mammalian

    Mutation

    without endogenous signal peptide (1-221bp)

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

11. JWW-1 human chimeric antibody

    Plasmid

    #66748

    Purpose

    Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-1 Heavy Chain Gamma
    JWW-1 Light Chain Kappa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    First 3 amino acids of rat light chain constant r…

    Promoter

    mEF1 and rEF1

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNK116

    Plasmid

    #219742

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D37E, V181I, S323M, M385K

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid

    #170537

    Purpose

    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 151-175

    Promoter

    CAG promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pETM6-4F2-mCherry

    Plasmid

    #73421

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4F2 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. ReactionC_pBIG1a_ATG7_ATG10

    Plasmid

    #190860

    Purpose

    Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG7 (ATG7 Human)
    ATG10 (ATG10 )
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-TagRFP658-P2A-GFP

    Plasmid

    #178971

    Purpose

    Expresses TagRFP658 and GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCV2_LacZ_sgRNA_2

    Plasmid

    #155093

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LZRS-BRaf-ERTm

    Plasmid

    #24949

    Depositor

    Paul Khavari

    Article

    Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

    Insert

    BRaf-ER (Esr1, BRAF Mouse, Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    V600E in BRAF

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExp-RBD-CHis

    Plasmid

    #195002

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ps-3HA-Beta2-PSCFP2-N

    Plasmid

    #114186

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA and PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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