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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,929 results for: nin

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Showing: 4481 - 4500 of 66929 results

  2. RH5-bio

    Plasmid
    #47780
    Purpose
    Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH5
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47780-plasmid-map-sequence-id-68799

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  3. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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  5. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Publication
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

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  10. pc DNA FRT TO- APP695

    Plasmid
    #114193
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    wild type splice variant 695: 695AA (2085bp, lack…
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114193-plasmid-map-sequence-id-224265

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  11. GFP-ZMYND11

    Plasmid
    #65402
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZMYND11 (ZMYND11 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pLSL

    Plasmid
    #51493
    Purpose
    T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Plant t-dna plasmid
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51493-plasmid-map-sequence-id-78588

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  17. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid
    #105802
    Purpose
    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-mRuby3; ORF2: TEV-mClover3
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105802-plasmid-map-sequence-id-203396

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  18. pEGFP TEM4 FL

    Plasmid
    #58893
    Purpose
    Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.
    Depositor
    Channing Der
    Publication
    Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TEM4 (ARHGEF17 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    L722F
    Promoter
    CMV
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58893-plasmid-map-sequence-id-341922

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  20. pAC84-pCR8-dCas9

    Plasmid
    #48218
    Purpose
    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A)
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48218-plasmid-map-sequence-id-69495

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Showing: 4481 - 4500 of 66929 results