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pJK227

Plasmid

#71126

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

GyrA intein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK136

Plasmid

#71122

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16-SeqFosC

Plasmid

#51432

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE C)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosC

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16-SeqFosD

Plasmid

#51433

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE D)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosD

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAF1-SeqFosC

Plasmid

#51428

Purpose

Artificial transcription factor with no activator domain targeting c-Fos (TALE C)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosC

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16-SeqFosA

Plasmid

#51429

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE A)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosA

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pTOPO-rDNA-NTS

Plasmid

#23992

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA NTS

Use

Bacterial cloning - topo

Mutation

None

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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NSUN6

Plasmid

#188060

Purpose

Bacterial expression plasmid for human NSUN6; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN6 (NSUN6 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT_Dn29-dCas9-P2A-GFP

Plasmid

#247165

Purpose

In vitro transcription of human codon optimized fusion of Dn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Promoter

mutated T7

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#188059

Purpose

Bacterial expression plasmid for human NSUN2; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN2 (NSUN2 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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TNPO1-shRNA

Plasmid

#86082

Purpose

A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sGluc

Plasmid

#186769

Purpose

An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAF112

Plasmid

#135920

Purpose

All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Type

Empty backbone

Use

Luciferase; Transposon

Expression

Mammalian

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p35S_GFP-GUS

Plasmid

#167122

Purpose

Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

GFP-GUS

Tags

mGFP5, GUS

Expression

Plant

Promoter

CaMV 35S

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
Stargazin-mCherry-FRB

Plasmid

#172443

Purpose

Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cacng2 (Cacng2 Mouse)

Tags

mCherry-HA-FRB

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET5s (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only