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pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Plasmid

#159800

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_055

Plasmid

#123920

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_049

Plasmid

#123946

Purpose

Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hThal5.10-2_hg18 landing pad destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-SHIV327C-HuA10-gp160

Plasmid

#123270

Purpose

Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

SHIV327C-Hu A10 Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG113

Plasmid

#133124

Purpose

pL0M-PU-NoP, promotorless region used as negative control

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

NoP promotorless region from plasmid pIJJ11268

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G13-L

Plasmid

#25758

Purpose

Entry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-FLAG-TAT-SIVmac251

Plasmid

#35979

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

SIV strain mac251

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJH104

Plasmid

#48262

Purpose

Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.

Depositor

Ronald Davis

Article

Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

Inserts

TEF1p 5' fragment
URA3 5' fragment
TEF1p 3' fragment

Use

Routine cloning vector

Mutation

From SK1 background. -343 to -1 relative to TEF1 …

Promoter

TEF1p

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCfB2991

Plasmid

#63643

Purpose

pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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myc-SpELL pBacPAK8

Plasmid

#15318

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCINTBeta1

Plasmid

#14067

Depositor

Richard Hynes

Article

Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

Insert

Chicken Beta 1 Integrin (ITGB1 Chicken)

Expression

Mammalian

Mutation

PstI-BglI fragment was blunt end cloned and EcoRI…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pJH124

Plasmid

#48259

Purpose

Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.

Depositor

Ronald Davis

Article

Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

Inserts

URA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter

Use

Routine cloning vector

Mutation

-242 upstream to URA3 ORF +495 and URA3 ORF from …

Promoter

Ag TEF1 promoter

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-SpEAF 60-251 pBacPAK8

Plasmid

#15321

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

EAF1 (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Mutation

N terminal deletion lacking first 59 amino acids …

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pHD Btl-pYtag(Scarlet) ScarlessDsRed

Plasmid

#244937

Purpose

Vector for tagging Breathless (Btl) with Scarlet-based pYtag biosensor. DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

Endogenous tagging

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE58

Plasmid

#247035

Purpose

IRF1 pathway reporter; lentiviral expression of IRF1 DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF1

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Torso

Plasmid

#244933

Purpose

CRISPR gRNA targeting the C-terminal end of Torso for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Torso C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Btl

Plasmid

#244934

Purpose

CRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Btl C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-EGFR

Plasmid

#244932

Purpose

CRISPR gRNA targeting the C-terminal end of EGFR for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting EGFR C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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