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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,321 results for: cas9

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Showing: 4521 - 4540 of 14321 results

  2. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  6. SpCas9-NRCH-ABE8e(V106W)

    Plasmid
    #198556
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198556-plasmid-map-sequence-id-388914

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  10. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid
    #133318
    Purpose
    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pasteurianus)
    Use
    Unspecified
    Promoter
    xylose
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    133318-plasmid-map-sequence-id-266833

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  14. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid
    #165450
    Purpose
    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter
    Depositor
    Elvir Becirovic
    Publication
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    U6 and short human rhodopsin promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    165450-plasmid-map-sequence-id-331048

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  20. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129026-plasmid-map-sequence-id-256128

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Showing: 4521 - 4540 of 14321 results