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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,563 results for: cycs

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Showing: 4521 - 4540 of 5563 results

  2. pSWD95Y14D

    Plasmid
    #159098
    Purpose
    CckA FRET Sensor with cyclic-di-GMP point mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    Expression
    Bacterial
    Mutation
    Mutated Y514 to D514
    Promoter
    Xylose
    Available Since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159098-plasmid-map-sequence-id-310723

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  5. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

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  8. GFP-X2DM

    Plasmid
    #115571
    Purpose
    GFP-X2 where both of the consensus motif's lysines are mutated to arginine
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. GFP-X2R2

    Plasmid
    #115570
    Purpose
    GFP-X2 where the second consensus motif's lysine is mutated to arginine
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available Since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115570-plasmid-map-sequence-id-226341

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  10. GFP-X2R1

    Plasmid
    #115569
    Purpose
    GFP-X2 where the first consensus motif's lysine is mutated to arginine
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available Since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115569-plasmid-map-sequence-id-226337

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  11. p5A1-T

    Plasmid
    #92017
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available Since
    Jan. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    92017-plasmid-map-sequence-id-201852

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  12. pMflT-o2

    Plasmid
    #101310
    Purpose
    Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101310-plasmid-map-sequence-id-198875

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  13. pMflT-o1

    Plasmid
    #101309
    Purpose
    Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101309-plasmid-map-sequence-id-198143

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  15. p5C3-T

    Plasmid
    #92022
    Purpose
    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92022-plasmid-map-sequence-id-190017

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  17. OpenLoopControl

    Plasmid
    #59895
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTR
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59895-plasmid-map-sequence-id-108253

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  18. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111431-plasmid-map-sequence-id-292191

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Showing: 4521 - 4540 of 5563 results