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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 4521 - 4540 of 18824 results

  1. pWS171

    Plasmid
    #90518
    Purpose
    Cas9 gap repair vector - LEU2
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90518-plasmid-map-sequence-id-288271

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  3. pDUAL SRBI (GFP)

    Plasmid
    #86979
    Purpose
    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    SCARB1 (SCARB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV/hPGK
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86979-plasmid-map-sequence-id-514761

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  4. Lenti-LbCpf1-Blast

    Plasmid
    #84751
    Purpose
    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    LbCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84751-plasmid-map-sequence-id-164465

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  5. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #69138
    Purpose
    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo
    Depositor
    Takuji Iwasato
    Publication
    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    Tags
    none
    Expression
    Mammalian
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69138-plasmid-map-sequence-id-165113

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  8. pRubiC-T2A-Cas9

    Plasmid
    #75347
    Purpose
    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    mCherry (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    75347-plasmid-map-sequence-id-232926

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  9. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75349-plasmid-map-sequence-id-266810

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  14. pRSV-PKI-v2

    Plasmid
    #45066
    Purpose
    Expression of PKI (PKA inhibitor)
    Depositor
    Richard Maurer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Expression
    Mammalian
    Promoter
    RSV
    Available since
    July 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45066-plasmid-map-sequence-id-62500

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  19. Anti-ProC [IPI-HPC-4] (Recombinant Antibody)

    Antibody
    #218108-rAb
    Purpose
    Anti-Protein C tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to EDQVDPRLIDGK sequence.
    Depositor
    Institute for Protein Innovation (IPI)
    Publication
    Stearns et al J Biol Chem. 1988 Jan 15;263(2):826-32.
    Recommended applications
    Western Blot
    Reactivity
    Human
    Source species
    Rabbit
    Isotype
    IgG
    Trial size
    Not available to purchase
    Available since
    May 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Institute for Protein Innovation (IPI)

  20. SpCas9-YE1-BE4max

    Plasmid
    #198546
    Purpose
    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198546-plasmid-map-sequence-id-390295

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Showing: 4521 - 4540 of 18824 results