We narrowed to 6,250 results for: nls

1. nuc-yHS1-M7A

   Plasmid

   #159167

   Purpose

   Nuclear labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   nuc-yHS1-M7A
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

   Promoter

   GPD

   Available Since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126778

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R691A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE3987

   Plasmid

   #137716

   Purpose

   Expresses dLbCas12a-RVRR-BE in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dLbCas12a-RVRR-BE
   

   Tags

   6xHis, APOBEC-1, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   G532A, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-NES-OptoJNKi

   Plasmid

   #89739

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Ro2

   Plasmid

   #24503

   Depositor

   Bruce Conklin

   Article

   Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

   Insert

   RASSL Ro2 (OPRK1 Human)
   

   Tags

   FLAG and NLS from prolactin

   Expression

   Mammalian

   Mutation

   mutations in extracellular loops 2 (see comments …

   Available Since

   June 16, 2010

   Availability

   Academic Institutions and Nonprofits only

7. JPF0522

   Plasmid

   #124013

   Purpose

   Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126777

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-GT-Dox-AIO-TetOn_ETV2_Down-Tandem

   Plasmid

   #241393

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ETV2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-ETV2; rtTA-T2A-mTagBFP2 (ETV2 Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE; CAG

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB758

    Plasmid

    #210033

    Purpose

    Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas7-P2A-Cas5-T2A-Cas8
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-FKBP-H3(pLxIS-PLPLR)

    Plasmid

    #221287

    Purpose

    Expression of H3 (pLxIS-PLPLR) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    H3 (pLxIS-PLPLR) (H3 Synthetic)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Mutation

    K to Q mutations made to inhibit NLS

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ230-D156R

    Plasmid

    #188542

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible lbcas12a-d156r entry c…

    Tags

    NLS

    Expression

    Plant

    Mutation

    D156R

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWP172

    Plasmid

    #198796

    Purpose

    Co-expression plasmid for the OLL neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pseb-3-NLS-gp41-1-C-intein-cGAL(AD)
    

    Expression

    Worm

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWP170

    Plasmid

    #198792

    Purpose

    Co-expression plasmid for the FLP, PVD neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    PY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWP095

    Plasmid

    #198789

    Purpose

    Co-expression plasmid for the CEP neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    PF35D11.1-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWP171

    Plasmid

    #198795

    Purpose

    Co-expression plasmid for the OLL neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Peat4prom9-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Expression

    Worm

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWP063

    Plasmid

    #198799

    Purpose

    Co-expression plasmid for the PHB neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Posm-10-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWP026

    Plasmid

    #198800

    Purpose

    Co-expression plasmid for the PHB neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Psrb-6-NLS-gp41-1-C-intein-cGAL(AD)
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWP097

    Plasmid

    #198790

    Purpose

    Co-expression plasmid for the CEP neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWP101

    Plasmid

    #198791

    Purpose

    Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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