We narrowed to 13,748 results for: TIM;
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Plasmid#225065PurposeExpresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanI-101)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCX-CD28 Y189F
Plasmid#223641PurposeRetroviral expression of mouse CD28 (Y189F) in mammalian cellsDepositorInsertCD28 (Cd28 Mouse)
UseRetroviral; mammalian expressionTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD80-CLIP
Plasmid#223590PurposeLentiviral expression of human CD80 with CLIP tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-CD80
Plasmid#223588PurposeLentiviral expression of human CD80 with SNAP tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CMV_oROS-G_LF(C199S)
Plasmid#216112PurposeExpresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.DepositorInsertoROS-G_LF(C199S)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-FKBPflex-ELL-CAAX
Plasmid#220076Purposean extra long linker attached to a CAAX domain with an mCherry tagDepositorInsertCAAX (HRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-G_LF(C199S)
Plasmid#216116PurposeEncodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.DepositorInsertoROS-G_LF(C199S)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-SF14P
Plasmid#209448Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from SF14P promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-kasOP*
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from kasOP* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro
Plasmid#189633PurposeLentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs librariesDepositorInserth7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2
Plasmid#189632PurposeLentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs librariesDepositorInserth7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-HiBIT-WDR5
Plasmid#200883PurposeBacterial expression of N-terminal HiBIT-tagged human WDR5 proteinDepositorInsertHiBIT-WDR5 (WDR5 Human)
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle.CC9
Plasmid#192931PurposeGateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterCMVAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc AspCas12a
Plasmid#182120PurposepET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (1-197)
Plasmid#188074PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationcodon-optimized ATG13 (1-197)PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA2
Plasmid#185007Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-IAP-del-gRNA1
Plasmid#185002Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertIAP-del-gRNA1 (Akp3 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMERVK9C-del-gRNA1
Plasmid#185004Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMERVK9C-del-gRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only