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pDEST_6xUAS:lyn-tagRFPt

Plasmid

#192354

Purpose

lyn-tagRFPt expression under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

lyn-tagRFPT

Tags

tagRFPT

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-SNAP-Smo

Plasmid

#31617

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

SNAP

Expression

Mammalian and Worm

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pICSL50008

Plasmid

#50314

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-BoxB-A114-N40

Plasmid

#50562

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-BoxB-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAN-PA2-RFP

Plasmid

#62248

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA2

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-CLIPf-WPRE

Plasmid

#104107

Purpose

Expresses CLIPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

CLIPf

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAct1IsGFP-1

Plasmid

#38775

Depositor

Peggy Lemaux

Article

Cho et al Plant Cell Rep. 2000 Nov;19(11):1084-108

Insert

Actin promoter and intron

Use

Plant transformation

Tags

nos 3' terminator and sGFP (S65T)

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pJMP12068

Plasmid

#222356

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and kanR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFF706

Plasmid

#61448

Purpose

Light-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pDawn

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pXWS10tetO

Plasmid

#160825

Purpose

10 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(10 X tetO)-t

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
BiDi-[mCherry2]mEGFP

Plasmid

#182874

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mEGFP, mCherry2

Expression

Mammalian

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pUCdeltaSS-ERE

Plasmid

#108217

Purpose

Yeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression

Depositor

Didier Picard, Keith Yamamoto

Article

Picard et al Nature 1990 Nov 8;348(6297):166-8.

Insert

ERE-CYC1-lacZ

Expression

Yeast

Promoter

CYC1

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a-dH2B

Plasmid

#105501

Purpose

Expression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression level

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH2B

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28CLY7

Plasmid

#21766

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pRSET-B-frSkyran-S

Plasmid

#182209

Purpose

Bacterial expression of frSkylan-S

Depositor

Shiro Suetsugu

Article

Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

Insert

frSkylan-S

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNXRVj-HACre

Plasmid

#78407

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
aur1R (aur1 Fission Yeast)

Expression

Yeast

Mutation

Gly 240 to Cys

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-SNAP-Smo

Plasmid

#31619

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pSEVA83-GG

Plasmid

#208016

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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