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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,016 results for: 338

Showing: 4561 - 4580 of 6016 results

  1. pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156912
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IGSF8 (IGSF8 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156912-plasmid-map-sequence-id-444413

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  2. pKT2-ACVR1-G328V-IRES-Katushka

    Plasmid
    #135002
    Purpose
    Expresses ACVR1 with a G328V mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Mendez et al Clin Cancer Res. 2020 Aug 1;26(15):4080-
    Insert
    Activin A receptor, type I (ACVR1) G328V (ACVR1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c.983G>T, p.G328V
    Promoter
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    135002-plasmid-map-sequence-id-271117

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  5. Capping Protein

    Plasmid
    #89950
    Purpose
    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.
    Inserts
    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    Use
    Tags
    6*His
    Expression
    Bacterial
    Mutation
    V222I
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89950-plasmid-map-sequence-id-183281

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  6. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Use
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187844-plasmid-map-sequence-id-379371

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  7. pTO_CHMP2B_LAP

    Plasmid
    #101848
    Purpose
    Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Flp-frt recombination, tet-inducible expression
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    silent mutation in Thr 104; A to T
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101848-plasmid-map-sequence-id-200235

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  9. pRS426-HsFECH

    Plasmid
    #174524
    Purpose
    Expresses human ferrochelatase (FECH) in yeast
    Depositor
    Oleh Khalimonchuk
    Article
    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.
    Insert
    FECH with yeast HEM15 promoter and terminator (FECH Budding Yeast, Human, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Lacks original mitochondrial targeting sequence (…
    Promoter
    HEM15 (yeast, 257 bp)
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    174524-plasmid-map-sequence-id-342886

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  16. Anti-GluN2C [N422/18R]

    Plasmid
    #177551
    Purpose
    Mammalian Expression Plasmid of anti-GluN2C/NR2C glutamate receptor (Rat). Derived from hybridoma N422/18.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-GluN2C/NR2C glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2c Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177551-plasmid-map-sequence-id-348762

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Showing: 4561 - 4580 of 6016 results