We narrowed to 26,927 results for: REV

1. pGBW-m4046508

   Plasmid

   #145419

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046357

   Plasmid

   #145417

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046539

   Plasmid

   #145389

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGBW-m4046345

   Plasmid

   #145381

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4046283

   Plasmid

   #145383

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046546

   Plasmid

   #145384

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046507

   Plasmid

   #145385

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pNB2629

   Plasmid

   #113394

   Purpose

   MET25p-MS2-RFP-CYC1 TT

   Depositor

   Suzanne Sandmeyer

   Article

   Clemens et al Virus Res. 2013 Feb;171(2):319-31. doi:

   Insert

   MS2 bacteriophage coat protein
   

   Tags

   red fluorescent protein (RFP), codon-optimized fo…

   Expression

   Yeast

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-10X-tRNA

   Plasmid

   #70123

   Purpose

   Gateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAs

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Insert

   10X-tRNA block
   

   Promoter

   None – SUP4 pol III promoter provided by insert

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pCfB2336

    Plasmid

    #67541

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. L284F Cus2p

    Plasmid

    #73059

    Purpose

    S. cerevisiae Cus2p gene with L284F Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Leucine 284 to Phenylalanine

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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12. D282N Cus2p

    Plasmid

    #73060

    Purpose

    S. cerevisiae Cus2p gene with D282N Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Asp 282 to Asn

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p11d-sctRPA

    Plasmid

    #102614

    Purpose

    Expression of S. cerevisiea RPA in E.coli

    Depositor

    Marc Wold

    Article

    Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

    Inserts

    RPA1 (RFA1 Budding Yeast)
    RPA2 (RFA2 Budding Yeast)
    RPA3 (RFA3 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    RFA2 has intron deleted

    Promoter

    T7

    Available Since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNZ18-CAH

    Plasmid

    #203159

    Purpose

    Replicates in E. coli, S. cerevisiae, and A. laidlawii

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZH82 pET28a-Rai1

    Plasmid

    #231651

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    rai1 (RAI1 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZH23 Dual G-less containing ySSA1 polyA

    Plasmid

    #231647

    Purpose

    yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    SSA1 polyA termination region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZH20 Dual G-less containing yCYC1 polyA

    Plasmid

    #231646

    Purpose

    yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    CYC1 polyA termination region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZH61 pLIB-8His3C-spt4

    Plasmid

    #231649

    Purpose

    express S.cerevisiae spt4 in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    spt4 (SPT4 Budding Yeast)
    

    Tags

    8His-HRV3C

    Expression

    Insect

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBF3060

    Plasmid

    #26853

    Purpose

    Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    GAL1

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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