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MT2a_C21A

Plasmid

#105697

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Cysteine 21 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K20A

Plasmid

#105698

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 20 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_S6A

Plasmid

#105699

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Serine 6 to Alanine

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK110

Plasmid

#110576

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP6 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK112

Plasmid

#110577

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP12b Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK113

Plasmid

#110578

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP32 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-fsaA

Plasmid

#102286

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

fsaA gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-adhE

Plasmid

#102290

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

adhE gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-3nmt1-mEOS3.2

Plasmid

#105166

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

kanMX6-3nmt1-mEOS3.2

Tags

3nmt1-mEOS3.2

Expression

Bacterial and Yeast

Promoter

3nmt1

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTC130

Plasmid

#91178

Purpose

BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALENs targeting tomato ANT1

Use

TALEN

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

Plasmid

#91874

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_1

Plasmid

#91159

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTC366

Plasmid

#91215

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0808

Plasmid

#91024

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0408

Plasmid

#91010

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0708

Plasmid

#91021

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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