We narrowed to 9,276 results for: ef1;
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CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX_lenti_osTir1_9Myc_P2A_Bsd
Plasmid#139746Purposelentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection markerDepositorInsertrice E3 ligase Tir1 (osTir1)
ExpressionMammalianPromoterEF1alphaAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgRNA-CLTA-mCherry
Plasmid#240637PurposeVector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.DepositorInsertpuro-T2A-mCherry
UseCRISPRExpressionMammalianAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX317-EGFP
Plasmid#115439PurposeConstitutive lentiviral expressionDepositorInsertEGFP
UseLentiviralTagsV5ExpressionMammalianPromoterEF1aAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-UniSAM
Plasmid#99866PurposeEncodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0DepositorInsertUniSAM-mCherry + U6-gRNA2.0
UseCRISPR and Synthetic Biology; Dcas9-sam activationExpressionMammalianMutationBbsI sites in CDS were ablated by consensus mutat…PromoterEF1aAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(WT)-FLAG
Plasmid#164972PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-COX4-dAPEX2
Plasmid#117177PurposePlasmid name in publication: pAAV-DIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAV and Cre/LoxExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-LACTB-dAPEX2
Plasmid#117180PurposePlasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAV and Cre/LoxExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IGK-dAPEX2-KDEL
Plasmid#117182PurposePlasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAVExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF/mt-(n1)StayGold
Plasmid#185823PurposeA soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.DepositorInsertmt-(n1)StayGold
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABE8e SpG Puro
Plasmid#235044PurposeAdenosine base editor 8e* with SpG nicking Cas9 makes A > G editsDepositorInsertABE8e, nCas9
UseCRISPR and LentiviralTagsP2A-PuroRPromoterEF1a coreAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS134
Plasmid#211505PurposeAAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertVenus
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingExpressionMammalianAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-TEAD1
Plasmid#229411PurposeLentiviral or overpexression of cDNADepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS133
Plasmid#211504PurposeAAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmTagBF2
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingExpressionMammalianAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS132
Plasmid#211503PurposeAAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seqDepositorInsertmCherry
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingExpressionMammalianAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only
